Browsing by Author "Feliciano, Amelia"
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- Hematological evaluation in males with obstructive Sleep apnea before and after positive airway pressurePublication . Feliciano, Amelia; Linhas, R.; Marçõa, R.; Cysneiros, A.; Martinho, C.; Reis, R.P.; Penque, Deborah; Pinto, P.; Bárbara, CristinaObstructive sleep apnea syndrome (OSAS) is a systemic inflammatory disease associated with cardiovascular consequences. Red blood cell distribution width (RDW), mean platelet volume (MPV), and platelet distribution width (PDW) are recognized biomarkers of cardiovascular morbidity/mortality. Limited data is available on the association between these parameters and OSAS severity and the relationship with positive airway pressure therapy (PAP). In this prospective study of male OSAS patients we analyzed hematological data in order to evaluate their value in predicting OSAS severity, the relationship with sleep parameters, and their behavior under PAP. Seventy-three patients were included (mean age 46.5 years), of which 36 were mild (49.3%), 10 moderate (13.7%), and 27 severe (37%). The mean RDW increased significantly with OSAS severity and showed a positive correlation with respiratory disturbance index and hypoxemic burdens. Additionally, a group of 48 patients (mean age 47.2 years) were submitted to PAP. After six months, red blood cell count, hemoglobin, hematocrit, and platelet count showed a significant decrease (p < 0.0001; p < 0.0001; p = 0.001; p < 0.0001; respectively). Concerning OSAS severity, these parameters also significantly decreased in mild patients (p = 0.003; p = 0.043; p = 0.020; p = 0.014; respectively) but only hemoglobin, hematocrit, and platelet count decreased in severe cases (p < 0.0001; p = 0.008; p = 0.018; respectively). This study demonstrated an association between RDW values and OSAS severity. Moreover, red cell and platelet parameters changed significantly after PAP, supporting its cardiovascular protective effect. RDW may become a simple/inexpensive blood biomarker, making it useful in prioritizing OSAS patients waiting for polysomnography, and red cell and platelet parameters could be useful in PAP follow up.
- Obstructive sleep apnea associated with Diabetes mellitus Type 2: a proteomic studyPublication . Vaz, Fátima; Valentim-Coelho, Cristina; Neves, Sofia; Feliciano, Amelia; Antunes, Marília; Pinto, Paula; Barbara, Cristina; Penque, DeborahBackground: We previously showed that Obstructive sleep apnea (OSA), a common public health concern causing deleterious cardiometabolic dysfunction, induced proteomic alterations in red blood cells (RBC) such as changes in the redox-oligomeric state of peroxiredoxin 2 (PRDX2)1-2. Herein, we aimed to investigate whether OSA patients with Type 2 Diabetes Mellitus before and after positive airway pressure (PAP) treatment present similar changes in the RBC antioxidant protein PRDX2 to better understand the molecular basic mechanisms associated with OSA and OSA outcomes. Methods: RBC samples from control snorers (n=22 being 3 diabetics) and OSA patients before and after six month of PAP-treatment (n=29 being 8 diabetics) were analysed by non-reducing western blot using antibody against PRDX2 or PRDXSO2/3 to measure the total and overoxidized levels of monomeric/dimeric/multimeric forms of PRDX2. Results: We confirmed previously data by showing that in OSA RBC the overoxidation on the monomeric forms of PRDX2 was higher compared to controls. After PAP treatment, this overoxidation decreased followed by an increase of multimeric-overoxidized forms of PRDX2 described to be associated with chaperone protective function. In contrast, the level of PRDX2 monomers in RBC diabetic OSA, although higher abundant its overoxidation level was much lower than those observed in OSA without comorbidity and did not significant change after treatment. Moreover, the level of PAP-induced PRDX2-overoxidized-multimers was also lower in these diabetic OSA patients. The level of overoxidized monomeric/dimeric forms of PRDX2 correlated negatively with levels of insulin / triglycerides and HbA1C, respectively. After PAP, the level of (overoxidized) PRDX2SO2/3 multimers correlated positively with adrenaline levels. Conclusions: The redox/oligomeric state of RBC PRDX2 that is regulated by overoxidation of the active cysteines was differentially modulated in diabetic OSA patients compared to OSA without this comorbidity. PAP-induced overoxidized oligo forms of PRDX2 that is associated with chaperone protective function showed decreased in OSA patients with diabetes. The clinical impact of these findings needs further investigation and validation.
- Proteomics in biomarkers discovery for Obstructive Sleep ApneaPublication . Feliciano, Amelia; Bozanic, Vesna; Sofia Carvalho, Ana; Almeida, Andreia; Vaz, Fatima
- Proteomics of Red Blood Cells from Patients with Obstructive Sleep ApneaPublication . Feliciano, Amelia; Bozanic, Vesna; Torres, Vukosava Milic; Matthiesen, Rune; Carvalho, Ana S.; Almeida, Andreia; Alexandre, Bruno; Vaz, Fátima; Malhotra, Atul; Pinto, Paula; Bárbara, Cristina; Penque, DeborahObstructive sleep apnea (OSA) is a common public health concern causing metabolic and cardiovascular consequences. Although OSA is a systemic disease, the molecular mechanisms and specific genes/proteins associated with such processes remain poorly defined.To identify dysregulated proteins that could be useful as candidate biomarkers of diagnosis/prognosis of OSA., is the aim of this study