Percorrer por autor "Correia, Susana"
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- First report of linezolid-resistant cfr-positive methicillin-resistant Staphylococcus aureus in humans in PortugalPublication . Silva, Vanessa; Almeida, Francisco; Silva, Adriana; Correia, Susana; Carvalho, José António; Castro, Ana Paula; Ferreira, Eugénia; Manageiro, Vera; Caniça, Manuela; Igrejas, Gilberto; Poeta, PatríciaLinezolid and vancomycin are the most reliable therapeutic agents to treat complicated soft tissue infections caused by multidrug-resistant methicillin-resistant Staphylococcus aureus (MRSA) strains. However, despite the fact that the prevalence of linezolid-resistant MRSA strains (LR-MRSA) remains overall low, in the past few years resistance to linezolid has been reported among human patients worldwide, imposing a public-health concern, in particular when associated with a mobile gene such as cfr [1]. The aim of this study was to identify LR-MRSA isolates recovered from infected diabetic foot ulcers and to characterise their antimicrobial resistance profiles and genetic lineage. (...)
- High prevalence of ESBL-producing Escherichia coli isolates among hemodialysis patients in Portugal - appearance of ST410 with the blaCTX-M-14 genePublication . Correia, Susana; Pacheco, Rui; Radhouania, Hajer; Dinize, João Carlos; Poncee, Pedro; Jones-Dias, Daniela; Caniça, Manuela; Igrejas, Gilberto; Poeta, PatríciaTen extended-spectrum β-lactamase–producing Escherichia coli isolates were detected among 121 fecal samples (8.3%) recovered from hemodialysis patients in Portugal. The isolates harbored the blaCTX-M-15, blaCTX-M-14a, and/or blaCTX-M-1 genes. A new sequence type, ST2229, was detected, and this study also reports, for the first time, ST410 CTX-M-14–producing isolates.
- Planning a One Health Case Study to Evaluate Methicillin Resistant Staphylococcus aureus and Its Economic Burden in PortugalPublication . Igrejas, Gilberto; Correia, Susana; Silva, Vanessa; Hébraud, Michel; Caniça, Manuela; Torres, Carmen; Gomes, Catarina; Nogueira, Fernanda; Poeta, PatríciaMethicillin-resistant Staphylococcus aureus (MRSA) is one of the most important multidrug-resistant nosocomial pathogens worldwide with infections leading to high rates of morbidity and mortality, a significant burden to human and veterinary clinical practices. The ability of S. aureus colonies to form biofilms on biotic and abiotic surfaces contributes further to its high antimicrobial resistance (AMR) rates and persistence in both host and non-host environments, adding a major ecological dimension to the problem. While there is a lot of information on MRSA prevalence in humans, data about MRSA in animal populations is scarce, incomplete and dispersed. This project is an attempt to evaluate the current epidemiological status of MRSA in Portugal by making a single case study from a One Health perspective. We aim to determine the prevalence of MRSA in anthropogenic sources liable to contaminate different animal habitats. The results obtained will be compiled with existing data on antibiotic resistant staphylococci from Portugal in a user-friendly database, to generate a geographically detailed epidemiological output for surveillance of AMR in MRSA. To achieve this, we will first characterize AMR and genetic lineages of MRSA circulating in northern Portugal in hospital wastewaters, farms near hospitals, farm animals that contact with humans, and wild animals. This will indicate the extent of the AMR problem in the context of local and regional human-animal-environment interactions. MRSA strains will then be tested for their ability to form biofilms. The proteomes of the strains will be compared to better elucidate their AMR mechanisms. Proteomics data will be integrated with the genomic and transcriptomic data obtained. The vast amount of information expected from this omics approach will improve our understanding of AMR in MRSA biofilms, and help us identify new vaccine candidates and biomarkers for early diagnosis and innovative therapeutic strategies to tackle MRSA biofilm-associated infections and potentially other AMR superbugs.
- Subproteomic signature comparison of in vitro selected fluoroquinolone resistance and ciprofloxacin stress in Salmonella Typhimurium DT104BPublication . Correia, Susana; Hébraud, Michel; Chafsey, Ingrid; Chambon, Christophe; Viala, Didier; Torres, Carmen; Caniça, Manuela; Capelo, José Luis; Poeta, Patrícia; Igrejas, GilbertoBackground: Fluoroquinolone resistance in nontyphoidal Salmonella is a situation of serious and international concern, particularly in S. Typhimurium DT104B multiresistant strains. Although known to be multifactorial, fluoroquinolone resistance is still far from a complete understanding. Methods: Subproteome changes between an experimentally selected fluoroquinolone-resistant strain (Se6-M) and its parent strain (Se6), and also in Se6-M under ciprofloxacin (CIP) stress, were evaluated in order to give new insights into the mechanisms involved. Proteomes were compared at the intracellular and membrane levels by a 2-DE~LC-MS/MS and a shotgun LC-MS/MS approach, respectively. Results: In total, 35 differentially abundant proteins were identified when comparing Se6 with Se6-M (25 more abundant in Se6 and 10 more abundant in Se6-M) and 82 were identified between Se6-M and Se6-M+CIP (51 more abundant in Se6-M and 31 more abundant under ciprofloxacin stress). Conclusion: Several proteins with known and possible roles in quinolone resistance were identified which provide important information about mechanism-related differential protein expression, supporting the current knowledge and also leading to new testable hypotheses on the mechanism of action of fluoroquinolone drugs.
- Vancomycin-resistant enterococci among haemodialysis patients in Portugal: Prevalence and molecular characterization of resistance, virulence and clonalityPublication . Correia, Susana; Ponce, Pedro; Jones-Dias, Daniela; Caniça, Manuela; Igrejas, Gilberto; Poeta, PatríciaIntroduction: Vancomycin-resistant enterococci (VRE) among haemodialysis patients has increased rapidly and, to date, there is no report of this incidence in Portugal. Methods: A total of 121 faecal samples were collected from haemodialysis patients, and then tested for VRE. Antimicrobial resistance, virulence and multilocus sequence typing (MLST) were studied. Results: VRE prevalence was 3.3%. Three VRE isolates, Enterococcus faecium, Enterococcus faecalis and Enterococcus raffinosus, were multi-resistant and vanA-positive. E. faecium and E. faecalis belonged to CC17 and CC2, respectively. Conclusion: Haemodialysis patients in Portugal are colonized with virulent, multi-resistant enterococci from high-risk clonal complexes, representing a public health concern.