Percorrer por autor "Allerberger, Franz"
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- Application of a real-time reverse transcription polymerase chain reaction for rapid detection of Escherichia coli in drinking water: an EU representative studyPublication . Gómez, Livia; Brandão, João; Navarro, Anna; Martines, Antonino; Sanseverino, Isabella; Porcel-Rodríguez, Elena; Marinov, Dimitar; Ableitner, Oksana; Allerberger, Franz; Atsma, Adrie; Briancesco, Rossella; Cenov, Arijana; Cíchová, Marianna; Coccia, Anna Maria; Libera, Simonetta Della; De Maeyer, Katrien; De Vries, Hendrik Jan; Elsinga, Goffe; Heijnen, Leo; Hokajärvi, Anna-Maria; Janská, Veronika; Hrabar, Jerko; Huse-Kutowska, Monika; Jozic, Slaven; Klatovsky, Stefanie; Kornschober, Christian; Kohls, Katharina; Krom, Adrie; Luden, Katrin; Mikula-Pratschke, Claudia; Pitkänen, Tarja; Poje, Mateja; Prokšová, Miloslava; Retelj, Matjaz; Rozwadowska, Beata; Rytkönen, Annastiina; Sarekoski, Anniina; Schlager, Sabine; Springer, Burkhard; Stojan, Iva; Stroobach, Eline; Šućić, Neven; Valério, Elisabete; Van Pelt, Gabi; Vranken, Jerom; Vrdoljak Tomaš, Ana; Vukić Lušić, Darija; Włos, Ewa; Wubbels, Gerhard; Ziombska, Maria; Zelenik, Katja; Ziuziakowski, Marcin; Lettieri, TeresaThe microbiological quality of water intended for human consumption in the EU is regulated by the recast Drinking Water Directive 2020/2184 (DWD), which sets strict parametric values for intestinal enterococci and Escherichia coli (E. coli), allowing no more than zero colony-forming units per 100 mL. Detection and enumeration of E. coli typically rely on culture-based reference methods or the most probable number approach, which require 1–2 days to produce results—potentially delaying timely action during contamination events. In contrast, molecular techniques can deliver results within hours. The DWD permits the use of alternative methods if they are as reliable as the reference method and developing and validating such methods requires multiple laboratory trials in line with ISO standard 16140-2. Following this, we conducted a representative EU study to validate a molecular method based on real-time reverse transcription polymerase chain reaction for rapid E. coli detection in drinking water. In a concerted action, the first of its kind for drinking water, nineteen laboratories across ten Member States participated. To ensure consistency, drinking water was artificially contaminated with E. coli. The alternative method showed slightly lower sensitivity than the reference method (91.1 % vs. 97.2 %) but delivered much faster results, making it a valuable screening tool. It can support rapid decision-making during contamination events, reducing the risk of waterborne outbreaks and protecting public health. For reliable routine performance, appropriate training in this alternative method is strongly recommended.
- Characterizing Antimicrobial Resistance in Clinically Relevant Bacteria Isolated at the Human/Animal/Environment Interface Using Whole-Genome Sequencing in AustriaPublication . Cabal, Adriana; Rab, Gerhard; Daza-Prieto, Beatriz; Stöger, Anna; Peischl, Nadine; Chakeri, Ali; Mo, Solveig Sølverød; Bock, Harald; Fuchs, Klemens; Sucher, Jasmin; Rathammer, Krista; Hasenberger, Petra; Stadtbauer, Silke; Caniça, Manuela; Strauß, Peter; Allerberger, Franz; Wögerbauer, Markus; Ruppitsch, WernerAntimicrobial resistance (AMR) is a public health issue attributed to the misuse of antibiotics in human and veterinary medicine. Since AMR surveillance requires a One Health approach, we sampled nine interconnected compartments at a hydrological open-air lab (HOAL) in Austria to obtain six bacterial species included in the WHO priority list of antibiotic-resistant bacteria (ARB). Whole genome sequencing-based typing included core genome multilocus sequence typing (cgMLST). Genetic and phenotypic characterization of AMR was performed for all isolates. Eighty-nine clinically-relevant bacteria were obtained from eight compartments including 49 E. coli, 27 E. faecalis, 7 K. pneumoniae and 6 E. faecium. Clusters of isolates from the same species obtained in different sample collection dates were detected. Of the isolates, 29.2% were resistant to at least one antimicrobial. E. coli and E. faecalis isolates from different compartments had acquired antimicrobial resistance genes (ARGs) associated with veterinary drugs such as aminoglycosides and tetracyclines, some of which were carried in conjugative and mobilizable plasmids. Three multidrug resistant (MDR) E. coli isolates were found in samples from field drainage and wastewater. Early detection of ARGs and ARB in natural and farm-related environments can identify hotspots of AMR and help prevent its emergence and dissemination along the food/feed chain.