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Detection of Rickettsia conorii strains in Portuguese dogs (Canis familiaris)

dc.contributor.authorAlexandre, Nuno
dc.contributor.authorSantos, AS
dc.contributor.authorBacellar, F
dc.contributor.authorBoinas, FJ
dc.contributor.authorNúncio, MS
dc.contributor.authorDe Sousa, R
dc.date.accessioned2012-02-27T11:14:43Z
dc.date.available2012-02-27T11:14:43Z
dc.date.issued2011-06
dc.description.abstractThis study reports a serological and molecular survey on the spotted fever group Rickettsia found in a group of 51 dogs with suspected tick-borne illness from the south of Portugal. Additionally, a prevalence of IgG antibodies to R. conorii was also estimated in a group of 400 healthy dogs from the same region. In the group of healthy dogs, the immunofluorescence test revealed that 154 (38.5%) of the 400 dogs had IgG antibodies reactive with R. conorii. The highest proportion of dogs (45%) with R. conorii antibodies was found in blood samples collected from October to December. Among the group suspected with tick-borne illness, 35 (62%) dogs showed to be seroreactive (IgG≥128) for antibodies against R. conorii, and the analysis of PCR-positive amplicons revealed that 5 dogs were infected with R. conorii Malish and 2 dogs were infected with R. conorii Israeli tick typhus strain. There was a higher prevalence of antibodies to R. conorii in the group of sick dogs, and the detection of R. conorii DNA in blood samples from this group points to their potential role as a reservoir and sentinel host helping to evaluate and characterize the distribution of circulating rickettsial strains.por
dc.identifier.citationTicks Tick Borne Dis. 2011 Jun;2(2):119-22. Epub 2011 Apr 16por
dc.identifier.issn1877-959X
dc.identifier.otherdoi:10.1016/j.ttbdis.2011.03.001
dc.identifier.urihttp://hdl.handle.net/10400.18/641
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1877959X11000239por
dc.subjectRickettsiapor
dc.subjectCãespor
dc.subjectR. conorii Malish strainpor
dc.subjectR. conorii Israeli tick typhus strainpor
dc.subjectDogpor
dc.subjectMediterranean spotted feverpor
dc.subjectSentinelpor
dc.subjectEstudos de Vectores e Doenças Infecciosaspor
dc.titleDetection of Rickettsia conorii strains in Portuguese dogs (Canis familiaris)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage122por
oaire.citation.startPage119por
oaire.citation.titleTicks and Tick-borne Diseasespor
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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