Publication
Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables
| dc.contributor.author | Jones-Dias, Daniela | |
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Ferreira, Eugénia | |
| dc.contributor.author | Barreiro, Paula | |
| dc.contributor.author | Vieira, Luís | |
| dc.contributor.author | Moura, Inês B | |
| dc.contributor.author | Caniça, Manuela | |
| dc.date.accessioned | 2017-02-17T11:44:24Z | |
| dc.date.available | 2017-02-17T11:44:24Z | |
| dc.date.issued | 2016-09-13 | |
| dc.description.abstract | The spread of antibiotic resistant bacteria throughout the food chain constitutes a public health concern. To understand the contribution of fresh produce in shaping antibiotic resistance bacteria and integron prevalence in the food chain, 333 antibiotic resistance Gram negative isolates were collected from organic and conventionally produced fruits (pears, apples, and strawberries) and vegetables (lettuces, tomatoes, and carrots). Although low levels of resistance have been detected, the bacterial genera identified in the assessed fresh produce are often described not only as environmental, but mostly as commensals and opportunistic pathogens. The genomic characterization of integron-harboring isolates revealed a high number of mobile genetic elements and clinically relevant antibiotic resistance genes, of which we highlight the presence of as mcr-1, qnrA1, bla GES-11, mphA, and oqxAB. The study of class 1 (n = 8), class 2 (n = 3) and class 3 (n = 1) integrons, harbored by species such as Morganella morganii, Escherichia coli, Klebsiella pneumoniae, led to the identification of different integron promoters (PcW, PcH1, PcS, and PcWTNG-10) and cassette arrays (containing drfA, aadA, cmlA, estX, sat, and bla GES). In fact, the diverse integron backbones were associated with transposable elements (e.g., Tn402, Tn7, ISCR1, Tn2 (*), IS26, IS1326, and IS3) that conferred greater mobility. This is also the first appearance of In1258, In1259, and In3-13, which should be monitored to prevent their establishment as successfully dispersed mobile resistance integrons. These results underscore the growing concern about the dissemination of acquired resistance genes by mobile elements in the food chain. | pt_PT |
| dc.description.sponsorship | DJ has received research funding from Fundação para a Ciência e a Tecnologia (FCT, grant number SFRH/BD/80001/2011). VM was supported by FCT fellowship (grant SFRH/BPD/77486/2011), financed by the European Social Funds (COMPETEFEDER) and national funds of the Portuguese Ministry of Education and Science (POPH-QREN). We thank the support of FCT grant number PEst-OE/AGR/UI0211/2011-2014 and UID/MULTI/00211/2013. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Front Microbiol. 2016 Sep 13;7:1400. doi: 10.3389/fmicb.2016.01400. eCollection 2016 | pt_PT |
| dc.identifier.doi | 10.3389/fmicb.2016.01400 | pt_PT |
| dc.identifier.issn | 1664-302X | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/4248 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Frontiers Media | pt_PT |
| dc.relation.publisherversion | http://journal.frontiersin.org/article/10.3389/fmicb.2016.01400/full | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | pt_PT |
| dc.subject | Fresh Produce | pt_PT |
| dc.subject | Agriculture | pt_PT |
| dc.subject | Antibiotic Resistance | pt_PT |
| dc.subject | Mobile Genetic Elements | pt_PT |
| dc.subject | Integrons | pt_PT |
| dc.subject | Resistência aos Antimicrobianos | pt_PT |
| dc.title | Architecture of Class 1, 2, and 3 Integrons from Gram Negative Bacteria Recovered among Fruits and Vegetables | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0211%2F2014/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00211%2F2013/PT | |
| oaire.citation.startPage | 1400 | pt_PT |
| oaire.citation.title | Frontiers in Microbiology | pt_PT |
| oaire.citation.volume | 7 | pt_PT |
| oaire.fundingStream | 5876 | |
| oaire.fundingStream | 5876 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | d3e4188b-642e-4ad7-9a4d-aeea65a89449 | |
| relation.isProjectOfPublication | 4641127c-7dd5-44f4-a3d1-d3c4d744ba51 | |
| relation.isProjectOfPublication.latestForDiscovery | 4641127c-7dd5-44f4-a3d1-d3c4d744ba51 |