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Publication
The Kidney Vero-E6 Cell Line: a Suitable Model to Study the Toxicity of Microcystins
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Advisor(s)
Abstract(s)
Microcystins (MCs) are toxins produced by cyanobacteria from water environments that can
induce acute and chronic effects on humans and animals, after ingestion/contact with contaminated
water. This group of cyclic heptapeptides comprises approximately 80 variants,
being microcystin-LR (MCLR) the most frequent and toxic variant. The studies of MCs effects on cell lines often lead to contradictory results, given the fact that
distinct MC toxicity endpoints (mainly cytotoxicity and genotoxicity) have been studied in
diverse cell lines (and cell clones) under distinct exposure conditions (different doses-ranges,
time of exposure, MCs variants, etc). In our work with Vero-E6 cells we tested MCLR (both
pure toxin and from cyanobacterial extracts of M. aeruginosa) within a wide range of concentrations
(1 nM- 200 μM), using several endpoints and methodologies (cytotoxicity, morphology,
genotoxicity, protein expression). In this chapter we will summarize our results and
discuss the utility of Vero-E6 cell line to evaluate the toxicological properties of MCLR.
Description
Keywords
Microcystin-LR Vero Cell Line Kidney Cytotoxicity Genotoxicity Tumor Promotion Água e Solo
Pedagogical Context
Citation
Carina Menezes, Elisabete Valério and Elsa Dias (2013). The Kidney Vero-E6 Cell Line: a Suitable Model to Study the Toxicity of Microcystins. In New Insights into Toxicity and Drug Testing, Dr. Sivakumar Gowder (Ed.), InTech, 29-48