Repository logo
 
Publication

Variation in the measurement of DNA damage by comet assay measured by the ECVAG† inter-laboratory validation trial

2010-03-02Journal article Open access
Simple item page
dc.contributor.authorForchhammer, Lykkepor
dc.contributor.authorJohansson, Clarapor
dc.contributor.authorLoft, Steffenpor
dc.contributor.authorMöller, Lennartpor
dc.contributor.authorGodschalk, Roger, W. L.por
dc.contributor.authorLangie, Sabine A. S.por
dc.contributor.authorJones, George D. D.por
dc.contributor.authorKwok, Rachel W. L.por
dc.contributor.authorCollins, Andrew R.por
dc.contributor.authorAzqueta, Amayapor
dc.contributor.authorPhillips, David H.por
dc.contributor.authorSozeri, Osmanpor
dc.contributor.authorStepnik, Maciejpor
dc.contributor.authorPalus, Jadwigapor
dc.contributor.authorVogel, Ullapor
dc.contributor.authorWallin, Hakanpor
dc.contributor.authorRoutledge, Michael N.por
dc.contributor.authorHandforth, Catherinepor
dc.contributor.authorAllione, Alessandrapor
dc.contributor.authorMatullo, Giuseppepor
dc.contributor.authorTeixeira, João Paulopor
dc.contributor.authorCosta, Solangepor
dc.contributor.authorRiso, Patriziapor
dc.contributor.authorPorrini, Marisapor
dc.contributor.authorMøller, Peterpor
dc.date.accessioned2011-06-01T16:39:14Zpor
dc.date.available2011-06-01T16:39:14Zpor
dc.date.issued2010-03-02por
dc.description.abstractThe comet assay has become a popular method for the assessment of DNA damage in biomonitoring studies and genetic toxicology. However, few studies have addressed the issue of the noted inter-laboratory variability of DNA damage measured by the comet assay. In this study, 12 laboratories analysed the level of DNA damage in monocyte-derived THP-1 cells by either visual classification or computer-aided image analysis of pre-made slides, coded cryopreserved samples of cells and reference standard cells (calibration curve samples). The reference standard samples were irradiated with ionizing radiation (0–10 Gy) and used to construct a calibration curve to calculate the number of lesions per 106 base pair. All laboratories detected dose–response relationships in the coded samples irradiated with ionizing radiation (1.5–7 Gy), but there were overt differences in the level of DNA damage reported by the different laboratories as evidenced by an inter-laboratory coefficient of variation (CV) of 47%. Adjustment of the primary comet assay end points by a calibration curve prepared in each laboratory reduced the CV to 28%, a statistically significant reduction (P < 0.05, Levene's test). A large fraction of the inter-laboratory variation originated from differences in image analysis, whereas the intra-laboratory variation was considerably smaller than the variation between laboratories. In summary, adjustment of primary comet assay results by reference standards reduces inter-laboratory variation in the level of DNA damage measured by the alkaline version of the comet assay.por
dc.identifier.citationMutagenesis. 2010 Mar;25(2):113-23. Epub 2009 Nov 12por
dc.identifier.issn0267-8357
dc.identifier.urihttp://hdl.handle.net/10400.18/100
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherOxford University Presspor
dc.relation.publisherversionhttp://mutage.oxfordjournals.org/content/25/2/113.full.pdf+htmlpor
dc.subjectSaúde ambientalpor
dc.subjectAr e Saúde Ocupacionalpor
dc.subjectToxicologiapor
dc.titleVariation in the measurement of DNA damage by comet assay measured by the ECVAG† inter-laboratory validation trialpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage123por
oaire.citation.startPage113por
oaire.citation.titleMutagenesispor
rcaap.rightsopenAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Variation in the measurement.pdf
Size:
254.25 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DSA - Artigos em revistas internacionais