Publication
Evaluation of the cyto- and genotoxicity of two types of cellulose nanomaterials using human intestinal cells and in vitro digestion simulation
| dc.contributor.author | Vital, Nádia | |
| dc.contributor.author | Cardoso, Maria | |
| dc.contributor.author | Kranendonk, Michel | |
| dc.contributor.author | Silva, Maria Joao | |
| dc.contributor.author | Louro, Henriqueta | |
| dc.date.accessioned | 2025-02-19T17:20:35Z | |
| dc.date.available | 2025-02-19T17:20:35Z | |
| dc.date.issued | 2024-12-24 | |
| dc.description.abstract | Emerging cellulose nanomaterials (CNMs) may have commercial impacts in multiple sectors, being their application particularly explored in the food sector. Thus, their potential adverse effects in the gastrointestinal tract should be evaluated before marketing. This work aimed to assess the safety of two CNMs (CNF–TEMPO and CMF–ENZ) through the investigation of their cytotoxicity, genotoxicity (comet and micronucleus assays), and capacity to induce reactive oxygen species in human intestinal cells, and their mutagenic effect using the <jats:italic>Hprt</jats:italic> gene mutation assay. Each toxicity endpoint was analysed after cells exposure to a concentration-range of each CNM or to its digested product, obtained by the application of a standardized static in vitro digestion method. The results showed an absence of cytotoxic effects in intestinal cells, up to the highest concentration tested (200 µg/mL or 25 µg/mL, for non-digested and digested CNMs, respectively). Of note, the cytotoxicity of the digestion control limited the top concentration of digested samples (25 µg/mL) for subsequent assays. Application of a battery of in vitro assays showed that CNF–TEMPO and CMF–ENZ do not induce gene mutations or aneugenic/clastogenic effects. However, due to the observed DNA damage induction, a genotoxic potential cannot be excluded, even though in vitro digestion seems to attenuate the effect. The lowest digested CNF–TEMPO concentration induced chromosomal damage in Caco-2 cells, leading to an equivocal outcome. Ongoing research on epigenotoxic effects of these CNMs samples may strengthen the lines of evidence on their safety when ingested, paving the way for their innovative application in the food industry. | eng |
| dc.description.sponsorship | This research was funded by the Portuguese Fundação para a Ciência e a Tecnologia, I.P. (FCT), through national funds PTDC/SAU–PUB/32587/2017 and PTDC/SAU–PUB/29481/2017. Research was co-funded by UIDB/00009/2020 (https://doi.org/10.54499/ UIDP/00009/2020) and UIDP/00009/2020 (https://doi.org/10.54499/ UIDB/00009/2020), both for the Centre for Toxicogenomics and Human Health—ToxOmics, FCT— Fundação para a Ciência e a Tecnologia. N.V. work was funded by Fundação para a Ciência e a Tecnologia, I.P., PhD grant number 2020.07168.BD. (https://doi.org/https:// doi.org/10.54499/2020.07168.BD). | |
| dc.identifier.citation | Arch Toxicol. 2025 Feb;99(2):575-596. doi: 10.1007/s00204-024-03911-2. Epub 2024 Dec 24 | |
| dc.identifier.doi | 10.1007/s00204-024-03911-2 | |
| dc.identifier.eissn | 1432-0738 | |
| dc.identifier.issn | 0340-5761 | |
| dc.identifier.pmid | 39718590 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10373 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Springer | |
| dc.relation | Toxicological profile of cellulose nanomaterials in human gastrointestinal cells | |
| dc.relation | A predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system | |
| dc.relation | Centre for Toxicogenomics and Human Health | |
| dc.relation | Centre for Toxicogenomics and Human Health | |
| dc.relation | Cellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials | |
| dc.relation.hasversion | https://link.springer.com/article/10.1007/s00204-024-03911-2 | |
| dc.relation.ispartof | Archives of Toxicology | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Cellulose nanomaterials | |
| dc.subject | In vitro digestion | |
| dc.subject | New Approach methodology | |
| dc.subject | Intestinal epithelial cells | |
| dc.subject | Micronucleus | |
| dc.subject | Reactive Oxygen Species | |
| dc.subject | Comet | |
| dc.subject | Environmental Genotoxicity | |
| dc.subject | Genotoxicidade Ambiental | |
| dc.title | Evaluation of the cyto- and genotoxicity of two types of cellulose nanomaterials using human intestinal cells and in vitro digestion simulation | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Toxicological profile of cellulose nanomaterials in human gastrointestinal cells | |
| oaire.awardTitle | A predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system | |
| oaire.awardTitle | Centre for Toxicogenomics and Human Health | |
| oaire.awardTitle | Centre for Toxicogenomics and Human Health | |
| oaire.awardTitle | Cellular and Molecular Mechanisms of Toxicity of Ingested Nanomaterials | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT//2020.07168.BD/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017/PTDC%2FSAU-PUB%2F29481%2F2017/PT | |
| oaire.citation.endPage | 596 | |
| oaire.citation.issue | 2 | |
| oaire.citation.startPage | 575 | |
| oaire.citation.title | Archives of Toxicology | |
| oaire.citation.volume | 99 | |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | Concurso para Financiamento de Projetos de Investigação Científica e Desenvolvimento Tecnológico em Todos os Domínios Científicos - 2017 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Vital | |
| person.familyName | Kranendonk | |
| person.familyName | Silva | |
| person.familyName | Louro | |
| person.givenName | Nádia | |
| person.givenName | Michel | |
| person.givenName | Maria Joao | |
| person.givenName | Henriqueta | |
| person.identifier | 117538 | |
| person.identifier | 157627 | |
| person.identifier.ciencia-id | FC1B-515E-3C03 | |
| person.identifier.ciencia-id | 7710-643D-97A3 | |
| person.identifier.ciencia-id | 721D-2BB1-7DB1 | |
| person.identifier.orcid | 0000-0002-3391-0268 | |
| person.identifier.orcid | 0000-0003-1362-0076 | |
| person.identifier.orcid | 0000-0002-6060-0716 | |
| person.identifier.orcid | 0000-0001-9744-7332 | |
| person.identifier.rid | H-8176-2013 | |
| person.identifier.scopus-author-id | 6603648823 | |
| person.identifier.scopus-author-id | 55944437600 | |
| person.identifier.scopus-author-id | 6507971479 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
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