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Characteristics of Haemophilus influenzae invasive isolates from Portugal following routine childhood vaccination against H. influenzae serotype b (2002-2010)

2014-04-04Journal article Restricted access
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dc.contributor.authorBajanca-Lavado, Maria Paula
dc.contributor.authorSimões, Alexandra
dc.contributor.authorBetencourt, Célia
dc.contributor.authorSá-Leão, Raquel
dc.contributor.authorPortuguese Group for the Study of Haemophilus influenzae invasive infection
dc.date.accessioned2015-10-08T12:59:30Z
dc.date.available2015-11-01T01:30:12Z
dc.date.issued2014-04-04
dc.description.abstractWe aimed to characterize Haemophilus influenzae invasive isolates recovered in Portugal over a 9-year period (2002-2010) following the inclusion of H. influenzae serotype b (Hib) conjugate vaccination in the National Immunization Program (NIP) in the year 2000 and compare the results with those obtained in a similar study from the pre-vaccination era (1989-2001) previously described by us. As part of a laboratory-based passive surveillance system, 144 invasive isolates obtained in 28 Portuguese hospitals were received at the National Reference Laboratory for Bacterial Respiratory Infections and were characterized. Capsular types and antibiotic susceptibility patterns were determined. The ftsI gene encoding PBP3 was sequenced for β-lactamase-negative ampicillin-resistant (BLNAR) isolates. Genetic relatedness among isolates was examined by multilocus sequencing typing (MLST). Most isolates (77.1%) were non-capsulated, a significant increase compared to the pre-vaccination era (19.0%, p < 0.001). Serotype b strains decreased significantly (from 81.0 to 13.2%, p < 0.001) and serotype f increased significantly (from 0.8 to 6.9%, p = 0.03). Ten percent of the isolates were β-lactamase producers, a value lower than that previously observed (26.9%, p = 0.005). Eight percent of all isolates were BLNAR. A high genetic diversity among non-capsulated isolates was found. By contrast, capsulated isolates were clonal. The implementation of Hib vaccination has resulted in a significant decline in the proportion of serotype b H. influenzae invasive disease isolates. Most episodes of invasive disease occurring in Portugal are now due to fully susceptible, highly diverse, non-capsulated strains. Given the evolving dynamics of this pathogen and the increase in non-type b capsulated isolates, continuous surveillance is needed.pt_PT
dc.description.sponsorshipThis work was supported by the National Institute of Health, Lisbon, Portugal.pt_PT
dc.identifier.citationEur J Clin Microbiol Infect Dis. 2014 Apr;33(4):603-10. doi: 10.1007/s10096-013-1994-6. Epub 2013 Oct 24.pt_PT
dc.identifier.doi10.1007/s10096-013-1994-6
dc.identifier.issn0934-9723
dc.identifier.urihttp://hdl.handle.net/10400.18/3186
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Linkpt_PT
dc.relation.publisherversionhttp://link.springer.com/article/10.1007%2Fs10096-013-1994-6pt_PT
dc.subjectHaemophilus Influenzaept_PT
dc.subjectInvasive Diseasept_PT
dc.subjectAntimicrobial Resistancept_PT
dc.subjectConjugate Vaccinept_PT
dc.subjectMLSTpt_PT
dc.subjectType bpt_PT
dc.subjectNon-Capsulatedpt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.titleCharacteristics of Haemophilus influenzae invasive isolates from Portugal following routine childhood vaccination against H. influenzae serotype b (2002-2010)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage610pt_PT
oaire.citation.startPage603pt_PT
oaire.citation.titleEuropean Journal of Clinical Microbiology and Infectious Diseasespt_PT
oaire.citation.volume33(4)pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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