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Occupational exposure to emerging cellulose nanomaterials and potential respiratory effects: uncovering exposure-effect relationships using in vitro systems

2022-09-27Conference object Restricted access
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dc.contributor.authorVentura, Célia
dc.contributor.authorPinto, Fátima
dc.contributor.authorVieira, Luís
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria João
dc.date.accessioned2022-11-28T12:33:18Z
dc.date.available2022-11-28T12:33:18Z
dc.date.issued2022-09-27
dc.description.abstractThe application of innovative cellulose nanomaterials (CNMs), particularly, cellulose micro- and nanofibrils (CMF, CNF) and nanocrystals (CNC), in technology and biomedicine has expanded in recent years. However, there is still a lack of reliable indicators to assess workers’ exposure, mainly by inhalation, and the associated health outcomes. This study aimed at identifying genotoxic, oxidative stress, or epigenetic endpoints that could be used as promising effect biomarkers to predict health outcomes from CNMs exposure, exploitable for human biomonitoring. Three well-characterized CNMs were used, at a low concentration range, to expose cell systems representative of the respiratory tract, namely, bronchial epithelial (BEAS-2B) cells or co-cultures of lung (A549) and macrophage-like (THP-1) cells. No induction of chromosomal instability was detected in co-cultures for all types of CNMs analysed through the cytokinesis-block micronucleus assay. Likewise, neither significant toxic effects nor oxidative stress was found. Interestingly, preliminary data showed differentially expressed microRNAs in BEAS-2B cells after exposure to CNC, but not to CMF or CNF, compared to untreated cells. Moreover, a profile of microRNAs associated with CNC exposure was identified. Overall, no major concern regarding CNMs genotoxicity and oxidative stress was raised. Epigenetic effects were uncovered for CNC and the identified miRNA profile has potential to be used as an effect biomarker for occupational biomonitoring. However, further investigation with longer exposures and validation of that profile using in vivo models must be conducted, to prove its value to assess early effects from CNMs exposure.pt_PT
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT/MCTES),through national funds(PIDDAC)(PTDC/SAU-PUB/32587/2017 and PTDC/SAU-PUB/29481/2017); ToxOmics - Centro de Investigação em Toxicogenómica e Saúde Humana - UIDP/00009/2020pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8339
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectCellulose Nanomaterialspt_PT
dc.subjectOccupational Exposurept_PT
dc.subjectOxidative Stresspt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectEpigeneticspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleOccupational exposure to emerging cellulose nanomaterials and potential respiratory effects: uncovering exposure-effect relationships using in vitro systemspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.citation.conferencePlaceLisboa, Portugalpt_PT
oaire.citation.titleAnnual Meeting International Society of Exposure Science (ISES), 25-29 september 2022pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameLouro
person.familyNameSilva
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier157627
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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