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Molecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugal

2017-03Journal article Restricted access
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dc.contributor.authorVilares, Anabela
dc.contributor.authorGargaté, Maria João
dc.contributor.authorFerreira, Idalina
dc.contributor.authorMartins, Susana
dc.contributor.authorGomes, João Paulo
dc.date.accessioned2018-03-06T15:32:11Z
dc.date.available2018-03-06T15:32:11Z
dc.date.issued2017-03
dc.description.abstractToxoplasma gondii is an apicomplexan parasite responsible for toxoplasmosis which infects all warm-blooded vertebrates, including mammals and birds. The majority of studies conducted in Europe have revealed that more than 80 % of strains isolated from human infections belong to genotype II, whereas genotypes I and III are responsible for a small number of cases. Atypical and recombinant strains are generally associated with more severe infections. In Portugal, there is a lack of data concerning genetic diversity as the classical typing studies in humans have never been performed. We aimed to determine the Sag2 and microsatellite-based (TUB2, TgM-A, W35, B17, B18) genotypes of T. gondii isolated from humans in Portugal, as well as to study their virulence in mice. We analyzed 48 strains from congenital and acquired toxoplasmosis collected during the last two decades. Sag2-based genotyping of T. gondii was achieved in all 48 strains where 35 (73 %) were classified as type II and 13 (27 %) were type I. The multilocus PCR of five microsatellites allowed the classification of 10 strains (21 %) as recombinant strains that had been previously identified as type II or I by Sag2 typing. Seven out of the 48 strains, including three type I, three recombinant, and one type I, were virulent in mice. This study constitutes the first evidence of recombinant strains circulating in Portugal in humans from congenital infection, highlighting the need for a better evaluation of these strains as their phenotype is still barely understood.pt_PT
dc.description.sponsorshipAnabela Vilares (SFRH/BD/75154/2010) was the recipient of a PhD fellowship supported by Fundação para a Ciência e Tecnologia.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationParasitol Res. 2017 Mar;116(3):979-985. doi: 10.1007/s00436-017-5374-5. Epub 2017 Jan 30.pt_PT
dc.identifier.doi10.1007/s00436-017-5374-5pt_PT
dc.identifier.issn0932-0113 (print)
dc.identifier.issn1432-1955 (online)
dc.identifier.urihttp://hdl.handle.net/10400.18/5213
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlagpt_PT
dc.relationGENETIC STUDY OF TOXOPLASMA GONDII STRAINS ISOLATED FROM HUMANS AND ANIMALS
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs00436-017-5374-5pt_PT
dc.subjectAnimalspt_PT
dc.subjectFemalept_PT
dc.subjectGenetic Variationpt_PT
dc.subjectGenotypept_PT
dc.subjectHumanspt_PT
dc.subjectMicept_PT
dc.subjectMice, Inbred ICRpt_PT
dc.subjectMicrosatellite Repeatspt_PT
dc.subjectPolymerase Chain Reactionpt_PT
dc.subjectToxoplasmapt_PT
dc.subjectToxoplasmosispt_PT
dc.subjectVirulencept_PT
dc.subjectCongenitalpt_PT
dc.subjectToxoplasma gondiipt_PT
dc.subjectRecombinantpt_PT
dc.subjectStrainpt_PT
dc.subjectPortugalpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleMolecular and virulence characterization of Toxoplasma gondii strains isolated from humans in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleGENETIC STUDY OF TOXOPLASMA GONDII STRAINS ISOLATED FROM HUMANS AND ANIMALS
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F75154%2F2010/PT
oaire.citation.endPage985pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage979pt_PT
oaire.citation.titleParasitology Researchpt_PT
oaire.citation.volume116pt_PT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication0f17c042-dbc5-49e6-8e73-22554709dbc4
relation.isProjectOfPublication.latestForDiscovery0f17c042-dbc5-49e6-8e73-22554709dbc4

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