Repository logo
 
Publication

Self-assembled Mannan Nanogel: Cytocompatibility and Cell Localization

2012-06-08Journal article Restricted access
Simple item page
dc.contributor.authorCarvalho, Vera
dc.contributor.authorCastanheira, Pedro
dc.contributor.authorMadureira, Pedro
dc.contributor.authorFerreira, Sílvia A.
dc.contributor.authorCosta, Carla
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorFaro, Carlos
dc.contributor.authorVilanova, Manuel
dc.contributor.authorGama, Miguel
dc.date.accessioned2013-02-12T10:36:20Z
dc.date.available2013-02-12T10:36:20Z
dc.date.issued2012-06-08
dc.description.abstractAmphiphilic mannan, produced by the Michael addition of hydrophobic 1-hexadecanethiol to vinyl methacrylated mannan, self-assembles in aqueous medium through hydrophobic interactions among alkyl chains. Resultant nanogel is stable, spherical, polydisperse, with 50-140 nm mean hydrodynamic diameter depending on the polymer degree of substitution, and nearly neutral negative surface charge. No cytotoxicity of mannan nanogel is detected up to about 0.4 mg/mL in mouse embryo fibroblast cell line 3T3 and mouse bone marrow-derived macrophages (BMDM) using cell proliferation, lactate dehydrogenase and Live/Dead assays. Comet assay, under the tested conditions, reveals no DNA damage in fibroblasts but possible in BMDM. BMDM internalize the mannan nanogel, which is observed in vesicles in the cytoplasm by confocal laser scanning microscopy. Confocal colocalization image analysis denotes that the entrance and exit of nanogel and FM 4-64 might occur by the same processes - endocytosis and exocytosis - in BMDM. Physicochemical characteristics, in vitro cytocompatibility and uptake of self-assembled mannan nanogel by mouse BMDM are great signals of the potential applicability of this nanosystem for macrophages targeted delivery of vaccines or drugs, acting as potential nanomedicines, always with the key goal of preventing and/or treating diseases.por
dc.identifier.citationJ Biomed Nanotechnol. 2012 Jun;8(3):473-81por
dc.identifier.issn1550-7033
dc.identifier.otherdoi:10.1166/jbn.2012.1408
dc.identifier.urihttp://hdl.handle.net/10400.18/1268
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Scientific publisherspor
dc.relation.publisherversionhttp://www.ingentaconnect.com/content/asp/jbn/2012/00000008/00000003/art00013?token=003e1298406720297d7634777b3a2b467a316a2d3b2b6d3f4e4b2588ed961dpor
dc.subjectColloidal Dispersionpor
dc.subjectConfocal Colocalizationpor
dc.subjectCytocompatibilitypor
dc.subjectImage Analysispor
dc.subjectMannan Nanogelpor
dc.subjectSel-assemblypor
dc.subjectUptakepor
dc.subjectAr e Saúde Ocupacionalpor
dc.titleSelf-assembled Mannan Nanogel: Cytocompatibility and Cell Localizationpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage481por
oaire.citation.startPage473por
oaire.citation.titleJournal of Biomedical Nanotechnologypor
oaire.citation.volume8(3)por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2012 J. Biomed. Nanotechnol. BRAGA.pdf
Size:
595.34 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DSA - Artigos em revistas internacionais