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Occupational secondhand smoke exposure may modify the proteoma expression of human nasal epithelium

2022-06Documento de conferência Acesso aberto
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dc.contributor.authorNeves, Sofia
dc.contributor.authorPacheco, Solange
dc.contributor.authorVaz, Fátima
dc.contributor.authorMartins, Inês
dc.contributor.authorJames, Peter
dc.contributor.authorSimões, Tânia
dc.contributor.authorPenque, Deborah
dc.date.accessioned2022-12-02T11:17:51Z
dc.date.available2022-12-02T11:17:51Z
dc.date.issued2022-06
dc.description.abstractThe tobacco is one of the biggest public health threats, smoking kills more than 7 million people/year worldwide and more than 890,000 are deaths resulting from exposure to Second Hand Smoke (SHS). In adults, SHS is associated to cardiovascular and respiratory diseases, including coronary heart disease and lung cancer, through pathological and molecular mechanisms not yet understood. In this study, we aimed to investigate the SHS effects on airway proteome in exposed workers. Nasal epithelium was collected from hospitality workers (non-smokers=40; smokers=12) long-term exposed and non-exposed to SHS at the workplace. Samples were analyzed by shotgun proteomics using an ESI-LQT Orbitrap XL mass spectrometer. The generated MS raw data was submitted to ‘PatternLab for Proteomics 4.0’ for peptide identification and relative quantification by label-free - extracted ion chromatograms (XIC). Golden rules were applied to obtain reliable data such as the inferred proteins must have at least one unique peptide identified to be considered and be detected in at least 80% of the cohort. Two proteins were found to be differentially expressed in the no-smokers exposed to SHS compared with the control: BPI fold-containing family A member 1 (BPIFA1) and Heat shock Protein Beta-1 (HSPB1). The first protein plays a role in the airway inflammatory response after exposure to irritants substances and the second is associated as a regulator of actin filament dynamics. Our findings support the indication that in non-smokers the prolonged exposure to SHS can lead to airway proteome modulation. When validated, the uncovered proteins can be promising candidates to “susceptibility/risk” and/or “response” biomarkers for SHS exposure.pt_PT
dc.description.sponsorshipAcknowledgements: Gulbenkian Institute of Science; Fundação para a Ciência e a Tecnologia (FCT); National Institute of Health Dr. Ricardo Jorge; Faculty of Sciences of the University of Lisbon; Protein Technology Laboratory - Department of Immunotecnology, Lund University, Sweden; Center of Toxicogenomics and Human Health.pt_PT
dc.description.versioninfo:eu-repo/semantics/draftpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8368
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectTobaccopt_PT
dc.subjectSecond Hand Smokept_PT
dc.subjectSHS Exposurept_PT
dc.subjectHuman Nasalpt_PT
dc.subjectEpitheliumpt_PT
dc.subjectGenómica Funcionalpt_PT
dc.subjectGenómica Funcional e Estruturalpt_PT
dc.titleOccupational secondhand smoke exposure may modify the proteoma expression of human nasal epitheliumpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceCaparica, Portugalpt_PT
oaire.citation.title8th International Caparica Conference on Analytical Proteomics, 18-21 July 2022pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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