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Detection of Echinococcus spp. and other taeniid species in lettuces and berries: Two international multicenter studies from the MEmE project

2025-01-06Artigo científico Acesso restrito
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datacite.subject.fosCiências Médicas
dc.contributor.authorUmhang, Gérald
dc.contributor.authorBastien, Fanny
dc.contributor.authorCartet, Alexandra
dc.contributor.authorAhmad, Haroon
dc.contributor.authorvan der Ark, Kees
dc.contributor.authorBerg, Rebecca
dc.contributor.authorBonelli, Piero
dc.contributor.authorDavidson, Rebecca K.
dc.contributor.authorDeplazes, Peter
dc.contributor.authorDeksne, Gunita
dc.contributor.authorGargate, Maria João
dc.contributor.authorVan der Giessen, Joke
dc.contributor.authorJamil, Naila
dc.contributor.authorJokelainen, Pikka
dc.contributor.authorKaramon, Jacek
dc.contributor.authorM'Rad, Selim
dc.contributor.authorMaksimov, Pavlo
dc.contributor.authorOudni-M'Rad, Myriam
dc.contributor.authorMuchaamba, Gillian
dc.contributor.authorOksanen, Antti
dc.contributor.authorPepe, Paola
dc.contributor.authorPoulle, Marie-Lazarine
dc.contributor.authorRinaldi, Laura
dc.contributor.authorSamorek-Pieróg, Małgorzata
dc.contributor.authorSantolamazza, Federica
dc.contributor.authorSantoro, Azzurra
dc.contributor.authorSantucciu, Cinzia
dc.contributor.authorSaarma, Urmas
dc.contributor.authorSchnyder, Manuela
dc.contributor.authorVillena, Isabelle
dc.contributor.authorWassermann, Marion
dc.contributor.authorCasulli, Adriano
dc.contributor.authorBoué, Franck
dc.date.accessioned2026-02-13T11:02:15Z
dc.date.available2026-02-13T11:02:15Z
dc.date.issued2025-01-06
dc.description.abstractCystic and alveolar echinococcosis are severe zoonotic diseases characterized by long asymptomatic periods lasting months or years. Viable Echinococcus spp. eggs released into the environment through the feces of canids can infect humans through accidental ingestion via hand-to-mouth contact or consumption of contaminated food or water. Both Echinococcus multilocularis and Echinococcus granulosus sensu lato are considered as foodborne parasites. However, when considering possible pathways of human infection, it appears that food and water-borne related variables do not significantly increase the risk of infection. Providing evidence-based data for the presence of DNA and, potentially, eggs in fresh produce is crucial in understanding foodborne transmission of Echinococcus spp. to humans. Two multicenter and multicountry studies were conducted within the One Health EJP framework to estimate the proportion of lettuces and berries contaminated by E. multilocularis, E. granulosus sensu lato, and other taeniid DNAs from a total of 12 European countries, Tunisia and Pakistan. A total of 1117 lettuces, 71 others vegetables, 300 strawberries, 130 blueberries and 50 others berries samples were collected and analysed by washing, sequential sieving and real-time PCRs. E. multilocularis DNA was detected in 1.2 % (7/570) of lettuce samples tested from the seven European endemic countries (Denmark, France, Germany, Latvia, the Netherlands, Poland and Switzerland) and in 2 % (2/100) from Pakistan. E. granulosus sensu lato DNA was identified in 1.3 % of lettuces (9/695) collected in five European endemic countries (France, Italy, Latvia, Poland and Portugal) and in 12 % (9/75) and 4 % (4/100) from Tunisia and Pakistan, respectively. All E. granulosus sensu lato samples were identified as E. granulosus sensu stricto (20/22), except for two identified as E. canadensis (2/22) from Latvia and Pakistan. Regarding berries, E. multilocularis DNA was detected in 5.4 % (n = 11/202) of strawberries, 7.3 % (6/82) of blueberries from the seven European endemic countries and 56 % (14/25) of blueberries from Pakistan. High contamination rates of E. granulosus sensu stricto were found outside of Europe, with 12.0 % (3/25) in blueberries from Pakistan and 81.3 %. (13/16) in strawberries from Tunisia. The total contamination rate of all taeniid species DNA in lettuces (5.3 %; 59/1117), others vegetables (5.6 %; 4/71) and berries (12.1 %; 58/480) suggests that the transfer of taeniid eggs from carnivore feces to food is not uncommon. Although we assume that eggs are the source of the DNA detected in this study, the viability of such eggs is unknown. The detection of Echinococcus species in lettuces and berries suggests a potential risk of foodborne human infection. The relative contribution of this risk remains to be estimated. Further studies on food and environmental contamination are necessary to cover different epidemiological contexts and social habits, leading to a better understanding of human infections by Echinococcus spp. eggs.eng
dc.description.abstractHighlights: -1117 lettuce and 480 berry samples were analysed for the detection of taeniid eggs DNA. -E. multilocularis DNA detected in 1.2% of lettuces, 5.4% of strawberries, 7.3% of blueberries in European endemic countries. -E. granulosus DNA detected in 1.3% of lettuces, 1.5% of strawberries, 1.3% of blueberries in European endemic countries.eng
dc.description.sponsorshipThis work was supported by funding from the European Union's Horizon 2020 Research and Innovation programme under grant agreement number 773830: One Health European Joint Programme (MEME project; https://onehealthejp.eu/jrp-meme/). This work was also partially supported by the European Commission's Single Market Programme (SMP Food) under the grant agreement no. 101144113: “Work programme 2023-2024 of EU European Reference Laboratory for the Parasites (EURLP)”. This work was also supported by research funding (grant PRG1209) from the Estonian Ministry of Education and Research (to US).
dc.identifier.citationInt J Food Microbiol. 2025 Feb 16:430:111059. doi: 10.1016/j.ijfoodmicro.2025.111059. Epub 2025 Jan 6
dc.identifier.doi10.1016/j.ijfoodmicro.2025.111059
dc.identifier.eissn1879-3460
dc.identifier.issn0168-1605
dc.identifier.pmid39787752
dc.identifier.urihttp://hdl.handle.net/10400.18/10926
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationPromoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards.
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0168160525000042?via%3Dihub
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEchinococcus granulosus sensu lato
dc.subjectEchinococcus multilocularis
dc.subjectEnvironmental Contamination
dc.subjectFoodborne Parasitic Infections
dc.subjectPathways of Transmission
dc.subjectRisk Factors
dc.subjectTaenia spp.
dc.subjectInfecções Sistémicas e Zoonoses
dc.titleDetection of Echinococcus spp. and other taeniid species in lettuces and berries: Two international multicenter studies from the MEmE projecteng
dc.typejournal article
dcterms.referenceshttps://www.sciencedirect.com/science/article/pii/S0168160525000042?via%3Dihub#ec0005
dcterms.referenceshttps://www.sciencedirect.com/science/article/pii/S0168160525000042?via%3Dihub#ec0010
dcterms.referenceshttps://www.sciencedirect.com/science/article/pii/S0168160525000042?via%3Dihub#ec0015
dspace.entity.typePublication
oaire.awardNumber773830
oaire.awardTitlePromoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards.
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/773830/EU
oaire.citation.issue430
oaire.citation.startPage111059
oaire.citation.titleInternational Journal of Food Microbiology
oaire.citation.volume16
oaire.fundingStreamH2020
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
relation.isProjectOfPublication94d118fb-33ce-49fa-b1ed-d5bddf63581d
relation.isProjectOfPublication.latestForDiscovery94d118fb-33ce-49fa-b1ed-d5bddf63581d

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