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Multiomics Substrates of Resistance to Emerging Pathogens? Transcriptome and Proteome Profile of a Vancomycin-Resistant Enterococcus faecalis Clinical Strain

2020-02Journal article Restricted access
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dc.contributor.authorPinto, Luís
dc.contributor.authorTorres, Carmen
dc.contributor.authorGil, Concha
dc.contributor.authorSantos, Hugo M.
dc.contributor.authorCapelo, José Luís
dc.contributor.authorBorges, Vítor
dc.contributor.authorGomes, João Paulo
dc.contributor.authorSilva, Catarina
dc.contributor.authorVieira, Luís
dc.contributor.authorPoeta, Patrícia
dc.contributor.authorIgrejas, Gilberto
dc.date.accessioned2021-04-05T17:20:22Z
dc.date.available2021-04-05T17:20:22Z
dc.date.issued2020-02
dc.description.abstractAntibiotic resistance and hospital acquired infections are on the rise worldwide. Vancomycin-resistant enterococci have been reported in clinical settings in recent decades. In this multiomics study, we provide comprehensive proteomic and transcriptomic analyses of a vancomycin-resistant Enterococcus faecalis clinical isolate from a patient with a urinary tract infection. The previous genotypic profile of the strain C2620 indicated the presence of antibiotic resistance genes characteristic of the vanB cluster. To further investigate the transcriptome of this pathogenic strain, we used whole genome sequencing and RNA-sequencing to detect and quantify the genes expressed. In parallel, we used two-dimensional gel electrophoresis followed by MALDI-TOF/MS (Matrix-assisted laser desorption/ionization-Time-of-flight/Mass spectrometry) to identify the proteins in the proteome. We studied the membrane and cytoplasm subproteomes separately. From a total of 207 analysis spots, we identified 118 proteins. The protein list was compared to the results obtained from the full transcriptome assay. Several genes and proteins related to stress and cellular response were identified, as well as some linked to antibiotic and drug responses, which is consistent with the known state of multiresistance. Even though the correlation between transcriptome and proteome data is not yet fully understood, the use of multiomics approaches has proven to be increasingly relevant to achieve deeper insights into the survival ability of pathogenic bacteria found in health care facilities.pt_PT
dc.description.sponsorshipL.P. was granted a PhD fellowship by Fundação para a Ciência e a Tecnologia and European Social Fund (SFRH/BD/81307/2011). This work was supported by the Associate Laboratory for Green Chemistry-LAQV, which is financed by national funds from Fundação para a Ciência e a Tecnologia/ MCTES (UID/QUI/50006/2019). This research is also a result of the GenomePT project (POCI-01-0145-FEDER- 022184), supported by COMPETE 2020—Operational Programme for Competitiveness and Internationalisation, Lisboa Portugal Regional Operational Programme (Lisboa, 2020), Algarve Portugal Regional Operational Programme (CRESC Algarve, 2020), under the PORTUGAL 2020 Partnership Agreement, through the EuropeanRegionalDevelopment Fund, andbyFundação para a Ciência e a Tecnologia.Work performed in University of La Rioja was supported by project SAF2016- 76571-R of Agencia Estatal de Investigatio´n and FEDER.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationOMICS. 2020 Feb;24(2):81-95. doi: 10.1089/omi.2019.0164.pt_PT
dc.identifier.doi10.1089/omi.2019.0164pt_PT
dc.identifier.issn1536-2310
dc.identifier.urihttp://hdl.handle.net/10400.18/7641
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebertpt_PT
dc.relationO PROTEOMA DE STRESS DE ENTEROCOCCUS RESISTENTES À VANCOMICINA: AVALIAÇÃO DAS ALTERAÇÕES NO GENOMA POR IDENTIFICAÇÃO E CARACTERIZAÇÃO DE PROTEÍNAS RELACIONADAS C/ RESISTÊNCIA A ANTIBIÓTICOS E VIRULÊNCIA
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.publisherversionhttps://www.liebertpub.com/doi/10.1089/omi.2019.0164pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectBacterial Proteinspt_PT
dc.subjectComputational Biologypt_PT
dc.subjectDrug Resistance, Microbialpt_PT
dc.subjectEnterococcus faecalispt_PT
dc.subjectGenome, Bacterialpt_PT
dc.subjectGenotypept_PT
dc.subjectGram-Positive Bacterial Infectionspt_PT
dc.subjectHumanspt_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.subjectMolecular Sequence Annotationpt_PT
dc.subjectPhylogenypt_PT
dc.subjectSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionizationpt_PT
dc.subjectStress, Physiologicalpt_PT
dc.subjectVancomycinpt_PT
dc.subjectVancomycin-Resistant Enterococcipt_PT
dc.subjectProteomept_PT
dc.subjectTranscriptomept_PT
dc.subjectMultiomicspt_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectProteomicspt_PT
dc.subjectTranscriptomicspt_PT
dc.subjectEnterococcuspt_PT
dc.subjectTranslational Researchpt_PT
dc.subjectBiomarkerspt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleMultiomics Substrates of Resistance to Emerging Pathogens? Transcriptome and Proteome Profile of a Vancomycin-Resistant Enterococcus faecalis Clinical Strainpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleO PROTEOMA DE STRESS DE ENTEROCOCCUS RESISTENTES À VANCOMICINA: AVALIAÇÃO DAS ALTERAÇÕES NO GENOMA POR IDENTIFICAÇÃO E CARACTERIZAÇÃO DE PROTEÍNAS RELACIONADAS C/ RESISTÊNCIA A ANTIBIÓTICOS E VIRULÊNCIA
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F81307%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FQUI%2F50006%2F2019/PT
oaire.citation.endPage95pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage81pt_PT
oaire.citation.titleOMICS: A Journal of Integrative Biologypt_PT
oaire.citation.volume24pt_PT
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb7caed21-15d0-4aa3-8385-5457b7bfdf53
relation.isProjectOfPublication5961359e-cce9-41c3-a5e6-b6170c28f652
relation.isProjectOfPublication.latestForDiscovery5961359e-cce9-41c3-a5e6-b6170c28f652

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