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Production and characterization of protein-rich extracts from the red macroalga Gelidium corneum and its industrial agar extraction residues

2024-02-09Journal article Open access
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datacite.subject.sdg14:Proteger a Vida Marinha
datacite.subject.sdg13:Ação Climática
dc.contributor.authorMateus, Marília
dc.contributor.authorMão de Ferro, Rita
dc.contributor.authorPinheiro, Helena M.
dc.contributor.authorMachado, Remígio
dc.contributor.authorR. da Fonseca, M. Manuela
dc.contributor.authorSapatinha, Maria
dc.contributor.authorPires, Carla
dc.contributor.authorMarmelo, Isa
dc.contributor.authorMarques, António
dc.contributor.authorNunes, Maria Leonor
dc.contributor.authorMotta, Carla
dc.contributor.authorCesário, M. Teresa
dc.date.accessioned2025-04-04T14:21:57Z
dc.date.available2025-04-04T14:21:57Z
dc.date.issued2024-02-09
dc.description.abstractThe red macroalga Gelidium corneum is a species commercially exploited for agar extraction, though with enormous potential for other industrial applications. This work aimed to produce protein-rich extracts from the crude alga and from residues of the industrial agar extraction process by applying different extraction and recovery procedures, and to characterize the obtained protein extracts. The sequential implementation of two water extractions followed by two alkaline extractions resulted in the recovery of 12.0 ± 1.2 % and 15.4 ± 0.9 % of the total protein contents of the crude alga and industrial residues, respectively. Two sequential water extractions assisted by Celluclast® and Alcalase® enzymes followed by two alkaline extractions recovered 52.1 ± 1.7 % and 36.5 ± 1.3 % of the protein contents of the crude alga and industrial residues, respectively. Extracts produced by these processes were characterized focusing on proximal composition, protein accessibility, nutrition and antioxidant potential, showing value as aquaculture feed ingredients. As all protein products revealed high carbohydrate contents and, aiming at enriching protein content, different protein precipitation methods were assessed, namely through ammonium sulfate precipitation and pH-shift protocols. The pH-shift method in sequential mode proved to be simple and fast with low reagent consumption. The purification of extracts by this precipitation mode allowed precipitating up to 72 % of the extracted protein. The results obtained suggest that protein concentrates extracted from Gelidium corneum appear to be promising sustainable sources for fish feed production, owing to their essential amino acid content, protein bioaccessibility and antioxidant properties.eng
dc.description.sponsorshipThe research leading to these results has received funding from Fundo Azul - DGPM, project FA-05-2017-033. Financial support was also received by national funds from FCT - Fundaçao ˜ para a Ciˆencia e a Tecnologia, I.P., in the scope of the project UIDB/04565/2020 and UIDP/04565/2020 and of the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. IM also acknowledges FCT I.P. for PhD grant (2020.04413.BD).
dc.identifier.citationAlgal Res. 2024 Feb 9;78:103420. doi:10.1016/j.algal.2024.103420
dc.identifier.doi10.1016/j.algal.2024.103420
dc.identifier.issn2211-9264
dc.identifier.urihttp://hdl.handle.net/10400.18/10486
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationInstitute for Bioengineering and Biosciences
dc.relationInstitute for Health and Bioeconomy
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S2211926424000328?via%3Dihub
dc.relation.ispartofAlgal Research
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectAlgae
dc.subjectComposição dos Alimentos
dc.subjectProtein
dc.subjectAquaculture
dc.subjectBioaccessibility
dc.subjectNutrition
dc.titleProduction and characterization of protein-rich extracts from the red macroalga Gelidium corneum and its industrial agar extraction residueseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.citation.startPage103420
oaire.citation.titleAlgal Research:Biomass, Biofuels and Bioproducts
oaire.citation.volume78
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublicationcf090881-a371-430c-9bfc-2f43275b41f8
relation.isProjectOfPublicationea242207-577b-433b-a1f6-8fc1a6385add
relation.isProjectOfPublication.latestForDiscoverycf090881-a371-430c-9bfc-2f43275b41f8

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