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Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal

2014-06Journal article Restricted access
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dc.contributor.authorSousa, Margarida
dc.contributor.authorSilva, Nuno
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorSilva, Filipe
dc.contributor.authorSargo, Roberto
dc.contributor.authorAlegria, Nuno
dc.contributor.authorBenito, Daniel
dc.contributor.authorGómez, Paula
dc.contributor.authorLozano, Carmen
dc.contributor.authorGómez-Sanz, Elena
dc.contributor.authorTorres, Carmen
dc.contributor.authorCaniça, Manuela
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2015-02-25T17:00:59Z
dc.date.available2015-02-25T17:00:59Z
dc.date.issued2014-06
dc.description.abstractAntibiotic resistance among wild animals represent an emerging public health concern. The objective of this study was to analyze the staphylococcal nasal microbiota in birds of prey and their content in antimicrobial resistance determinants. Nasal samples from 16 birds of prey were collected, swabs were dipped and incubated into BHI broth [6.5% NaCl] and later seeded on manitol salt agar and oxacillin-resistance screening agar base media. Staphylococcal colonies were isolated from both media and were identified by biochemical and molecular methods. Susceptibility testing to 18 antimicrobial agents was performed by disk-diffusion method. Six of the 16 tested animals carried staphylococci (37.5%) and 7 isolates of the following species were recovered: Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus sciuri rodentium, Staphylococcus cohnii urealitycum, and Staphylococcus gallinarum. The S. aureus isolate was penicillin-resistant (with blaZ gene) but methicillin-susceptible and was ascribed to spa-type t012, sequence-type ST30 and agr-type III. The S. epidermidis isolate carried blaZ, mecA, mrs(A/B), mphC, tet(K), drfA, and fusC genes, ica operon, and was typed as ST35. The genes ant6′-Ia, tet(K), tet(L), dfrG, cat221, cat194, and cat223 were detected in S. saprophyticus or S. gallinarum isolates. Birds of prey seem to be a natural reservoir of S. aureus and coagulase-negative staphylococci resistant to multiple antibiotics. Due to the convergence between habitats, the contact between wildlife, other animals and humans is now more common and this involves an increased possibility of interchange of these microorganisms in the different ecosystems.por
dc.description.sponsorshipMargarida Andrade Sousa has her Ph.D. fellowship granted by FCT (Fundac¸a˜o para a Cieˆncia e Tecnologia, Portugal) with the reference SFRH/BD/87302/2012 co- financed by the Social European Union Found and the Operational Program for Human Potential and National Board for Reference and Strategic Programs (POPH/QREN), Portugal. Nuno Silva was supported by ‘‘Programa Cieˆncia 2008’’ co-financed by POPH/QREN Type 4.2: Employment Promotion Scientific subsidized by the European Social Fund and National Funds of the Ministry of Science and Technology for Higher Education (MCTES). Part of this work was supported by Project SAF2012- 35474 from the Ministerio de Economía y Competitividad of Spain and the Fondo Europeo de Desarrollo Regional (FEDER).por
dc.identifier.citationVet Microbiol. 2014 Jul 16;171(3-4):436-40. doi: 10.1016/j.vetmic.2014.02.034. Epub 2014 Mar 3por
dc.identifier.doi10.1016/j.vetmic.2014.02.034
dc.identifier.issn0378-1135
dc.identifier.urihttp://hdl.handle.net/10400.18/2956
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationSAF2012- 35474por
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0378113514001242por
dc.subjectResistência aos Antibióticospor
dc.subjectCoagulase-negative staphylococcipor
dc.subjectBirds of Preypor
dc.subjectIca Operonpor
dc.subjectS. aureuspor
dc.subjectS. epidermidispor
dc.subjectPortugalpor
dc.titleAntimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugalpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage440por
oaire.citation.startPage436por
oaire.citation.titleVeterinary Microbiologypor
oaire.citation.volume171(3-4)por
rcaap.rightsembargoedAccesspor
rcaap.typearticlepor

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