Publicação
In vitro and in vivo assessment of nanoceria biocompatibility for their safe use in nervous system applications
| dc.contributor.author | Fernández-Bertólez, Natalia | |
| dc.contributor.author | Martínez, Luisa | |
| dc.contributor.author | Ramos-Pan, Lucía | |
| dc.contributor.author | Touzani, Assia | |
| dc.contributor.author | Costa, Carla | |
| dc.contributor.author | Laffon, Blanca | |
| dc.contributor.author | Valdiglesias, Vanessa | |
| dc.date.accessioned | 2026-02-23T12:49:19Z | |
| dc.date.available | 2026-02-23T12:49:19Z | |
| dc.date.issued | 2025-03-15 | |
| dc.description.abstract | Nanoceria, or cerium dioxide nanoparticles (CeO NP), are increasingly employed in a number of industrial and commercial applications. Hence, the environmental presence of these nanoparticles is growing progressively, enhancing the global concern on their potential health effects. Recent studies suggest that nanoceria may also have promising biomedical applications particularly in neurodegenerative and brain-related pathologies, but studies addressing their toxicity, and specifically on the nervous system, are still scarce, and their potential adverse effects and action mechanism are not totally understood yet. The objective of this work was to assess the biological behaviour of CeO NP in vitro in human nervous systems cells, and in vivo in Drosophila melanogaster to characterize their safety for exposed individuals and verify their suitability to be further employed in diagnosis and treatment of nervous system disorders. Cell cycle alterations, late apoptosis rate and DNA damage (comet and γH2AX assays), were determined in neuronal SH-SY5Y and glial A172 cells treated with nanoceria. Moreover, the survival rate, morphological changes and behavioural alterations were analysed in D. melanogaster individuals chronically exposed to CeO NP. The results obtained from the in vitro assessment showed that the nanoceria generally presented a good biocompatibility with scarce cyto- or genotoxic effects, essentially depending the exposure time and cell type, and being restricted to the longer exposure periods. Nevertheless, decrease in adult size and alterations observed in the larval crawling in the in vivo assays highlight the need of further investigations before establishing clinical uses of nanoceria. | por |
| dc.description.abstract | Highlights: - The environmental presence of nanoceria is growing due to multiple applications. - Nanoceria have promising biomedical uses, but their neurotoxicity is not discarded. - This work aimed to assess the biological behaviour of CeO2 NP in vitro and in vivo. - Nanoceria presented a good biocompatibility with scarce cyto- or genotoxic effects. - Effects observed in vivo highlight the need of further research before clinical use. | eng |
| dc.description.sponsorship | This research was funded by Ministry of Science and Innovation: MCIN/AEI/10.13039/501100011033 (Grant PID2020-114908GA-I00) and Xunta de Galicia (ED431B 2022/16). Funding for open access charge: Universidade da Coruña/CISUG. | |
| dc.identifier.citation | J Hazard Mater. 2025 Mar 15:486:137041. doi: 10.1016/j.jhazmat.2024.137041. Epub 2024 Dec 31 | |
| dc.identifier.doi | 10.1016/j.jhazmat.2024.137041 | |
| dc.identifier.issn | 0304-3894 | |
| dc.identifier.pmid | 39754877 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10987 | |
| dc.language.iso | en | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation.hasversion | https://www.sciencedirect.com/science/article/pii/S0304389424036227?via%3Dihub | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Biocompatibility | |
| dc.subject | Cerium Dioxide Nanoparticles | |
| dc.subject | Cytotoxicity | |
| dc.subject | Drosophila melanogaster | |
| dc.subject | Genotoxicity | |
| dc.subject | Nervous System | |
| dc.subject | Genotoxicidade Ambiental | |
| dc.subject | Toxicologia | |
| dc.title | In vitro and in vivo assessment of nanoceria biocompatibility for their safe use in nervous system applications | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | 137041 | |
| oaire.citation.title | Journal of Hazardous Materials | |
| oaire.citation.volume | 486 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |