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Overview of in vitro approaches to investigate chemical contaminants intestinal transport

2023-09Conference object Restricted access
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dc.contributor.authorAlves de Sousa, B.
dc.contributor.authorFaria, M.
dc.contributor.authorMiralles, B.
dc.contributor.authorAlvito, Paula
dc.date.accessioned2024-01-03T14:07:32Z
dc.date.available2024-01-03T14:07:32Z
dc.date.issued2023-09
dc.description.abstractThe occurrence of chemical contaminants in food products is a serious threat to public health and can lead from mild gastroenteritis to fatal liver, kidney and neurological syndromes. The main route of exposure to chemical contaminants is ingestion, with the intestine being the first organ to come into contact with these compounds. In vitro intestinal cell assays may constitute a useful tool to predict the impact of chemical contaminants on the intestinal barrier. Differentiated Caco-2 cells cultivated in porous Transwell inserts, as an in vitro model to evaluate the intestinal transport of food chemical contaminants, is recurrent. These toxicants can be transported via transcellular (transport that occurs through the epithelial cell) or paracellular (transport that occurs in between cells) pathway. This study aims to make an overview of in vitro approaches to investigate chemical contaminants intestinal transport and associated pathways. A bibliographical search was carried out in two databases of scientific publications, “PUBMED” and “WEB OF SCIENCE”, using the keywords “food”, “chemical contaminants”, “cell transport”, in scientific publications from 1999 to 2021. Forty publications were used for data extraction. In sum, Caco-2 cells were the most frequently used model for the evaluation of the food contaminants intestinal transport. In a few works, this cell line was combined with the human HT-29 cells. In other studies, specifically evaluating the implication of membrane transporters, Madin-Darby canine kidney (MDCK) cells were used. HTC-8 intestinal organoids were also reported and gastric absorption of food toxicants was described in a single work using NCI-N87 cells. The use intestinal mucosa explants mounted in Ussing chambers, in studies of food contaminants intestinal transport in farmed animals, was also reported, although in much less extension. The transcellular route was the most reported pathway for chemical contaminants, with paracellular being less referred (mycotoxins and chloropropanols). Further studies concerning absorption and transport of chemical contaminants are needed to better characterize its permeability across epithelial membranes which will contribute to a comprehensive understanding of its impact on human health. In addition, their frequent co-occurrence raises an additional number of questions that can only be answered using high throughput in vitro methods.pt_PT
dc.description.sponsorshipThanks are due to the EarlyMyco project (PTDC/MEDTOX/28762/2017), and to CESAM (UIDP/50017/2020+UIDB/50017/2020+LA/P/0094/2020), funded by FCT/MCTES through national funds.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8821
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.relationCentre for Environmental and Marine Studies
dc.subjectToxicologiapt_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectContaminantes Alimentarespt_PT
dc.subjectSaúde Humanapt_PT
dc.titleOverview of in vitro approaches to investigate chemical contaminants intestinal transportpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardTitleCentre for Environmental and Marine Studies
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMED-TOX%2F28762%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50017%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0094%2F2020/PT
oaire.citation.conferencePlaceCampinas, Brasilpt_PT
oaire.citation.title5th International Conference on Food Contaminants (ICFC2023), 4-6 September 2023pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isProjectOfPublication2635814e-db0a-43c5-b745-a2a237efb72e
relation.isProjectOfPublication4e22de31-8fc5-4b15-b980-3fd0edf5515d
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relation.isProjectOfPublicationbc5e46b5-0da4-4ee2-97e0-bf2ab95a5870
relation.isProjectOfPublication.latestForDiscovery2635814e-db0a-43c5-b745-a2a237efb72e

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