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Identification and detection of a novel point mutation in the Chitin Synthase gene of Culex pipiens associated with diflubenzuron resistance

2020-05-01Journal article Open access
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dc.contributor.authorFotakis, E.A.
dc.contributor.authorMastrantonio, V.
dc.contributor.authorGrigoraki, L.
dc.contributor.authorPorretta, D.
dc.contributor.authorPugglioli, A.
dc.contributor.authorChaskopoulu, A.
dc.contributor.authorOsório, H.
dc.contributor.authorWeill, M.
dc.contributor.authorBellini, R.
dc.contributor.authorUrbanelli, S.
dc.contributor.authorVontas, J.
dc.date.accessioned2021-03-05T09:51:49Z
dc.date.available2021-03-05T09:51:49Z
dc.date.issued2020-05-01
dc.description.abstractBackground: Diflubenzuron (DFB) is one of the most used insecticides in mosquito larval control including that of Culex pipiens, the proven vector of the recent West Nile Virus epidemics in Europe. Two mutations (I1043L and I1043M) in the chitin synthase (CHS) putative binding site of DFB have been previously reported in Cx. pipiens from Italy and associated with high levels of resistance against this larvicide. Methodology/principal findings: Here we report the identification of a third mutation at the same I1043 position of the CHS gene resulting in the substitution of Isoleucine to Phenylalanine (I1043F). This mutation has also been found in agricultural pests and has been functionally validated with genome editing in Drosophila, showing to confer striking levels (>15,000 fold) of DFB resistance. The frequency of the I1043F mutation was found to be substantially higher in Cx. pipiens mosquitoes surviving DFB doses largely exceeding the recommended field dose, raising concerns about the future efficient use of this insecticide. We monitored the presence and frequency of DFB mutations in Cx. pipiens mosquitoes from several Mediterranean countries, including Italy, France, Greece, Portugal and Israel. Among the Cx. pipiens populations collected in Northern Italy all but one had at least one of the three DFB mutations at allele frequencies reaching 93.3% for the I1043M, 64.8% for the I1043L and 10% for the I1043F. The newly reported I1043F mutation was also identified in two heterozygote individuals from France (4.2% allelic frequency). In contrast to Italy and France, no DFB resistant mutations were identified in the Cx. pipiens mosquitoes sampled from Greece, Portugal and Israel. Conclusions/significance: The findings of our study are of major concern for mosquito control programs in Europe, that rely on the use of a limited number of available larvicides, and highlight the necessity for the development of appropriate Insecticide Resistance Management (IRM) programs, to ensure the sustainable use of DFB.pt_PT
dc.description.abstractAuthor summary: Diflubenzuron is one of the main larvicides used for the control of the West Nile Virus vector Culex pipiens in the Mediterranean. However, the efficiency of control is now under threat due to the selection of insecticide resistance. Two point mutations were previously identified at the Chitin synthase and shown to confer low and high levels of resistance and a diagnostic was developed to monitor the trait. This study reports the identification of a third mutation associated with high levels of diflubenzuron resistance in Italy. This mutation was also detected in France, whereas no resistance mutations were found in Cx. pipiens mosquitoes sampled from Greece, Portugal and Israel. The findings are of major concern for mosquito control programs in S. Europe, which rely on the use of a limited number of larvicides.pt_PT
dc.description.sponsorshipE.A. F. was supported by the General Secretariat for Research and Technology (GSRT) and the Hellenic Foundation for Research and Innovation (HFRI) in the context of the action “1st Proclamation of Scholarships from ELIDEK for PhD Candidates” (Scholarship Code: 532). JV and RB received funds from European Union’s Horizon (INFRAVEC) research and innovation programme under grant agreement No 731060 (https://infravec2.eu/).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPLoS Negl Trop Dis. 2020 May 1;14(5):e0008284. doi: 10.1371/journal.pntd.0008284. eCollection 2020 May.pt_PT
dc.identifier.doi10.1371/journal.pntd.0008284pt_PT
dc.identifier.issn1935-2735
dc.identifier.urihttp://hdl.handle.net/10400.18/7322
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPublic Library of Sciencept_PT
dc.relationResearch Infrastructures for the control of vector-borne diseases
dc.relation.publisherversionhttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0008284pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectIdentification and Detectionpt_PT
dc.subjectMutationpt_PT
dc.subjectChitin Synthase Genept_PT
dc.subjectCulex Pipienspt_PT
dc.subjectDiflubenzuronpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleIdentification and detection of a novel point mutation in the Chitin Synthase gene of Culex pipiens associated with diflubenzuron resistancept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleResearch Infrastructures for the control of vector-borne diseases
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/731060/EU
oaire.citation.issue5pt_PT
oaire.citation.startPagee0008284pt_PT
oaire.citation.titlePLoS Neglected Tropical Diseasespt_PT
oaire.citation.volume14pt_PT
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationf3035277-fb14-4956-ab59-b6a9d871d600
relation.isProjectOfPublication.latestForDiscoveryf3035277-fb14-4956-ab59-b6a9d871d600

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