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Whole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Isolated from Human Bloodstream Infections

2025-02-20Artigo científico Acesso aberto
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dc.contributor.authorSabença, Carolina
dc.contributor.authorRivière, Rani
dc.contributor.authorCosta, Eliana
dc.contributor.authorSousa, Sara
dc.contributor.authorCaniça, Manuela
dc.contributor.authorSilva, Vanessa
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorTorres, Carmen
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2026-02-05T15:00:02Z
dc.date.available2026-02-05T15:00:02Z
dc.date.issued2025-02-20
dc.description(This article belongs to the Special Issue Antimicrobial Resistance in Bloodstream Infections: Epidemiology and Application of Rapid Diagnostics)
dc.description.abstractKlebsiella pneumoniae is a Gram-negative bacterium commonly associated with bloodstream infections (BSIs), which can lead to severe clinical outcomes, especially in immunocompromised individuals or patients with underlying health conditions. The increasing prevalence of K. pneumoniae that produces extended-spectrum β-lactamases (ESBL) poses a significant challenge for treatment and infection control, necessitating a swift diagnostic approach and tailored antimicrobial therapy to improve patient outcomes. A total of 32 K. pneumoniae isolates were recovered from BSIs from December 2021 to August 2022. Whole-genome sequencing (WGS) was performed on the 14 ESBL-producing isolates. All ESBL isolates carried the blaCTX-M-15 gene, together with other β-lactamase-encoding genes (blaTEM-1, blaSHV-28, blaSHV-26, or blaOXA-1). Three of the isolates also carried the blaKPC-3 gene. Resistance genes to quinolones, sulfonamides, tetracycline, aminoglycosides, and chloramphenicol were also detected. We can conclude that the presence of ESBL-producing isolates among K. pneumoniae of BSIs raises concerns, since these enzymes limit the available treatment options, and future research must include studies on alternative therapies for dealing with resistant bacterial infections and developing new approaches to disease treatment.eng
dc.description.sponsorshipThis work was supported by the projects UI/00772 and LA/P/0059/2020 funded by the Portuguese Foundation for Science and Technology (FCT) and by the FCT/MCTES (LA/P/0008/2020 DOI:10.54499/LA/P/0008/2020, UIDP/50006/2020 DOI: 10.54499/UIDP/50006/2020, and UIDB/50006/2020 DOI: 10.54499/UIDB/50006/2020) through national funds.
dc.identifier.citationPathogens. 2025 Feb 20;14(3):205. doi: 10.3390/pathogens14030205
dc.identifier.doi10.3390/pathogens14030205
dc.identifier.eissn2076-0817
dc.identifier.pmid40137690
dc.identifier.urihttp://hdl.handle.net/10400.18/10816
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationAssociate Laboratory for Animal and Veterinary Sciences
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relation.hasversionhttps://www.mdpi.com/2076-0817/14/3/205
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectKlebsiella pneumoniae
dc.subjectbloodstream infections
dc.subjectextended-spectrum ?-lactamases
dc.subjectwhole-genome sequencing
dc.subjectResistência aos Antimicrobianos
dc.titleWhole-Genome Sequencing of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Isolated from Human Bloodstream Infectionseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAssociate Laboratory for Animal and Veterinary Sciences
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0059%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0008%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.citation.issue3
oaire.citation.startPage205
oaire.citation.titlePathogens
oaire.citation.volume14
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublication93de5af7-dedc-4696-ae11-592584b020a8
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