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Genetic Relatedness and Diversity of Staphylococcus aureus from Different Reservoirs: Humans and Animals of Livestock, Poultry, Zoo, and Aquaculture

2020-09-03Journal article Open access
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dc.contributor.authorSalgueiro, Vanessa
dc.contributor.authorManageiro, Vera
dc.contributor.authorBandarra, Narcisa M.
dc.contributor.authorFerreira, Eugénia
dc.contributor.authorClemente, Lurdes
dc.contributor.authorCaniça, Manuela
dc.date.accessioned2021-04-02T15:43:04Z
dc.date.available2021-04-02T15:43:04Z
dc.date.issued2020-09-03
dc.descriptionThis article belongs to the Special Issue Deciphering the Crosswalk Dimension of Antibiotic Resistance between Different Reservoirspt_PT
dc.description.abstractThe main aim of this study was the characterization of antibiotic resistance mechanisms in 82 Staphylococcus aureus strains isolated from humans and animals. Antibiotic susceptibility testing was performed on all S. aureus isolates accordingly, and antibiotic-resistant genes were investigated by genotypic methods. The genetic diversity of S. aureus was studied through spa, multilocus sequence typing (MLST), and agr typing methods. The majority of S. aureus from human sources were resistant to cefoxitin (and harbor the mecA gene) and fluoroquinolones, whereas only four strains of S. aureus from animal sources revealed resistance to ciprofloxacin. In the set of S. aureus isolated from humans, the most frequent spa, MLST, and agr group were t032, ST22, and I, respectively. In strains from animal origin the most common spa, MLST, and agr group found were t2383, ST398, and III/not typable, respectively. S. aureus from humans and animals were identified either in clonal complexes CC5, CC30, and CC398, suggesting that they have the same putative founder in their evolution. Considering the three CCs encompassing strains from human and animal reservoirs with different spa-types, we can hypothesize that this might reflect an adaptation to different phylogenetic lineages in those reservoirs (host species) probably associated to genetic diversification of pre-existing strains.pt_PT
dc.description.sponsorshipVanessa Salgueiro had her Ph.D. fellowship granted by FCT (Fundação para a Ciência e a Tecnologia) with the reference SFRH/BD/133100/2017 co-financed by European Social Fund and the Operational Program for Human Capital (POCH), Portugal. We thank the following laboratories for sending human S. aureus isolates and laboratory records to the NRL-AMR-HAI: CHLC (M. Pinto), CHLO (E. Gonçalves), CHMA (H. Silva), CHMT (E. C. Cortes), CHON (A. Vicente), CHS (J. Duarte), CHSJ (J. Guimarães), CHTMAD (E. Costa), CHTS (M. E. Dias), CHUC (L. Albuquerque), CHVNG (A. Lira), HDFF (A. P. Vasco), HGO (J. Diogo), HH, Faial (J. Sachicumbi), H. Luz (P. Paixão), Hemobiolab (A. S. Jorge), HSMMB (A. Rocha); IPO Coimbra (F. Valido), LAC/A&D (M. Garcez), LAC/GAIA (A. Marques), and Laclibe (A. Gonçalves).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicroorganisms. 2020 Sep 3;8(9):1345. doi: 10.3390/microorganisms8091345.pt_PT
dc.identifier.doi10.3390/microorganisms8091345pt_PT
dc.identifier.issn2076-2607
dc.identifier.urihttp://hdl.handle.net/10400.18/7626
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationDecrypting the diversity of microbiome in aquaculture
dc.relation.publisherversionhttps://www.mdpi.com/2076-2607/8/9/1345pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMRSApt_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectST398pt_PT
dc.subjectAnimalspt_PT
dc.subjectHuman Isolatespt_PT
dc.subjectPortugalpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleGenetic Relatedness and Diversity of Staphylococcus aureus from Different Reservoirs: Humans and Animals of Livestock, Poultry, Zoo, and Aquaculturept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleDecrypting the diversity of microbiome in aquaculture
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F133100%2F2017/PT
oaire.citation.issue9pt_PT
oaire.citation.startPage1345pt_PT
oaire.citation.titleMicroorganismspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationb16cfc8b-bb9b-43b9-9b11-2c49a0856050
relation.isProjectOfPublication.latestForDiscoveryb16cfc8b-bb9b-43b9-9b11-2c49a0856050

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