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Tobacco Smoke Occupational Exposure: Biomarkers of Biological Damage

2011-10-17Conference object Restricted access
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dc.contributor.authorSimões, Tânia
dc.contributor.authorMilic, Vukosava D.
dc.contributor.authorPacheco, S.A.
dc.contributor.authorAguiar, Fátima
dc.contributor.authorGomes, Filomena
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorVital, Nádia
dc.contributor.authorAntunes, Susana
dc.contributor.authorCharro, Nuno
dc.contributor.authorBruno, Alexandre
dc.contributor.authorVaz, Fátima
dc.contributor.authorLopes, C.
dc.contributor.authorMarçal, N.
dc.contributor.authorFragoso, E.
dc.contributor.authorProença, C.
dc.contributor.authorSekera, M.
dc.contributor.authorHagenfeld, Manuela
dc.contributor.authorSilva, Maria João
dc.contributor.authorAlmeida, A.B.
dc.contributor.authorPenque, Deborah
dc.contributor.authorRuivo, P.
dc.date.accessioned2012-02-15T18:18:33Z
dc.date.available2012-02-15T18:18:33Z
dc.date.issued2011-10-17
dc.descriptionWork perfomed by Instituto Nacional Saúde Doutor Ricardo Jorge, Laboratório de Análises de Dopagem, Instituto do Desporto de Portugal, Clínica Universitária Pneumologia, Faculdade Medicina Lisboa, Hospital Santa Mariapor
dc.description.abstractHigh concentration of toxic substances emanated from tobacco smoke in entertainment places such as restaurants, bars and nightclubs may compromise indoor air quality (IAQ) generating environments of likelihood health risk. Their employees, particularly those exposed to second-hand smoke, are at increased risk for developing chronic respiratory diseases such as chronic obstructive pulmonary disease (COPD), asthma and lung cancer. Objectives In this work, we aimed at evaluating relationships between occupational ETS exposure, biological damage (DNA or proteome alterations) and putative respiratory dysfunctions. Materials and Methods A group of restaurants located in Lisbon has been studied, in which fine particles (smaller than 2.5µm; PM2.5), indicative of environmental tobacco smoke (ETS) contamination, were measured. After informed consent, workers were evaluated for acute exposure to ETS based on cotinine levels in urine and clinically evaluated for their respiratory health by spirometry measurements and chestpiece auscultation. Effects of ETS exposure on genotoxic lesions were evaluated by measuring DNA/chromosomes breaks in peripheral blood lymphocytes and buccal mucosa cells. Effects of ETS exposure on plasma proteins is being studied using a 2D-DIGE-MALDI-TOF/TOF approach. To achieve that goal, global proteome characterization is being carried based on the same individual plasma samples collected for genotoxic studies and were pooled according to previous criteria. Results Results have confirmed higher respirable particle levels in smoking-designated areas of those entertainment places, indicating an ETS contamination. Leukocytes from ETS-exposed-workers presented lower levels of genotoxic-induced damage in comparison with non-exposed workers, suggesting an ETS-induced stress adaption response in exposed-workers. By proteomics, we are now to investigating those workers for putative alterations on their plasma proteome to provide additional insights on the adaptative response mechanisms that might be activated by ETS exposition. Conclusion Altogether, this study provides information on indoor air quality of Lisbon smoking entertainment places, in particular ETS contamination, and may provide biomarker candidates for occupational ETS-exposure which might precede respiratory diseases on their employees.por
dc.description.sponsorshipWork supported by Fundação Calouste Gulbenkian, Administração Central do Sistema de Saúde, FCT/PolyAnnual Funding Program and FEDER/SaudeXXI Program, Portugal. VDM, SP,NC,BA and TS, are recipients of FCT fellowships.por
dc.identifier.urihttp://hdl.handle.net/10400.18/583
dc.language.isoengpor
dc.publisherInstituto Nacional de Saúde Doutor Ricardo Jorge, IPpor
dc.subjectEnvironmental Tobbaco Smokepor
dc.subjectDNA Damagepor
dc.subjectIndoor Air Qualitypor
dc.subjectSmokingpor
dc.subjectGenotoxicidade Ambientalpor
dc.subjectAr e Saúde Ocupacionalpor
dc.subjectBiomarkerspor
dc.subjectOccupational exposurepor
dc.subjectChronic Respiratory Diseasespor
dc.titleTobacco Smoke Occupational Exposure: Biomarkers of Biological Damagepor
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePorto, Portugalpor
oaire.citation.titleInternational Conference on Occupational and Environmental Health 2011 & Workshop Biomarkers & Human Exposure to Nanoparticles, 17-19th October 2011por
rcaap.rightsrestrictedAccesspor
rcaap.typeconferenceObjectpor

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