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Identification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extracts

2023-03-04Journal article Open access
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dc.contributor.authorMalmir, Maryam
dc.contributor.authorLima, Katelene
dc.contributor.authorPóvoas Camões, Sérgio
dc.contributor.authorManageiro, Vera
dc.contributor.authorDuarte, Maria Paula
dc.contributor.authorPaiva Miranda, Joana
dc.contributor.authorSerrano, Rita
dc.contributor.authorMoreira da Silva, Isabel
dc.contributor.authorSilva Lima, Beatriz
dc.contributor.authorCaniça, Manuela
dc.contributor.authorSilva, Olga
dc.date.accessioned2023-03-20T14:31:25Z
dc.date.available2023-03-20T14:31:25Z
dc.date.issued2023-03-04
dc.descriptionThis article belongs to the Special Issue Discovery of Bioactive Ingredients from Natural Products III.
dc.description.abstractThe leaves of Asphodelus bento-rainhae subsp. bento-rainhae, an endemic Portuguese species, and Asphodelus macrocarpus subsp. macrocarpus have been used as food, and traditionally as medicine, for treating ulcers, urinary tract, and inflammatory disorders. The present study aims to establish the phytochemical profile of the main secondary metabolites, together with the antimicrobial, antioxidant and toxicity assessments of both Asphodelus leaf 70% ethanol extracts. Phytochemical screenings were conducted by the TLC and LC-UV/DAD-ESI/MS chromatographic technique, and quantification of the leading chemical classes was performed by spectrophotometric methods. Liquid-liquid partitions of crude extracts were obtained using ethyl ether, ethyl acetate, and water. For in vitro evaluations of antimicrobial activity, the broth microdilution method, and for the antioxidant activity, the FRAP and DPPH methods were used. Genotoxicity and cytotoxicity were assessed by Ames and MTT tests, respectively. Twelve known compounds including neochlorogenic acid, chlorogenic acid, caffeic acid, isoorientin, p-coumaric acid, isovitexin, ferulic acid, luteolin, aloe-emodin, diosmetin, chrysophanol, and β-sitosterol were identified as the main marker compounds, and terpenoids and condensed tannins were found to be the major class of secondary metabolites of both medicinal plants. The ethyl ether fractions demonstrated the highest antibacterial activity against all the Gram-positive microorganisms, (MIC value of 62 to 1000 µg/mL), with aloe-emodin as one of the main marker compounds highly active against Staphylococcus epidermidis (MIC value of 0.8 to 1.6 µg/mL). Ethyl acetate fractions exhibited the highest antioxidant activity (IC50 of 800 to 1200 µg/mL, respectively). No cytotoxicity (up to 1000 µg/mL) or genotoxicity/mutagenicity (up to 5 mg/plate, with/without metabolic activation) were detected. The obtained results contribute to the knowledge of the value and safety of the studied species as herbal medicines.pt_PT
dc.description.sponsorshipThis research was funded by the Foundation for Science and Technology (FCT, Portugal) through national funds FCT/MCTES to iMed.ULisboa (UIDP/04138/2020, UIDB/04138/2020) and MEtRICs (UIDP/04077/2020, UIDB/04077/2020) research projects, as well as doctoral scholarship (SFRH/BD/125310/2016) granted to the first author.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMolecules. 2023 Mar 4;28(5):2372. doi: 10.3390/molecules28052372.pt_PT
dc.identifier.doi10.3390/molecules28052372pt_PT
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10400.18/8574
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMechanical Engineering and Resource Sustainability Center
dc.relationMechanical Engineering and Resource Sustainability Center
dc.relationDiscovery of new antimicrobial drugs active against resistant pathogen agents: The case study of Portuguese Asphodelus species
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/28/5/2372pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAloe-emodinpt_PT
dc.subjectAntimicrobialpt_PT
dc.subjectAntioxidantpt_PT
dc.subjectAsphodeluspt_PT
dc.subjectChemical Profilept_PT
dc.subjectHerbal Medicinespt_PT
dc.subjectPreclinical Safetypt_PT
dc.subjectStaphylococcus Epidermidispt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleIdentification of Marker Compounds and In Vitro Toxicity Evaluation of Two Portuguese Asphodelus Leaf Extractspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMechanical Engineering and Resource Sustainability Center
oaire.awardTitleMechanical Engineering and Resource Sustainability Center
oaire.awardTitleDiscovery of new antimicrobial drugs active against resistant pathogen agents: The case study of Portuguese Asphodelus species
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04077%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04077%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F125310%2F2016/PT
oaire.citation.issue5pt_PT
oaire.citation.startPage2372pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume28pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationed49c1c5-9ca2-46a0-bc88-450edc6a078c
relation.isProjectOfPublicationeeea3256-1b3a-4b50-a399-e612ad441012
relation.isProjectOfPublication20adaf32-4cf7-43fb-9018-1e84fd0f0878
relation.isProjectOfPublication.latestForDiscoveryeeea3256-1b3a-4b50-a399-e612ad441012

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