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Genotypic diversity of clinical Coxiella burnetii isolates from Portugal based on MST and MLVA-typing

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dc.contributor.authorSantos, A.S.
dc.contributor.authorTilburg, J.J.H.C.
dc.contributor.authorBotelho, A.
dc.contributor.authorBarahona, M.J.
dc.contributor.authorNúncio, M.S.
dc.contributor.authorNabuurs-Franssen, M.H.
dc.contributor.authorKlaassen, C.H.W.
dc.date.accessioned2012-10-25T16:30:16Z
dc.date.available2012-10-25T16:30:16Z
dc.date.issued2012
dc.description.abstractGenotypic diversity of clinical Coxiella burnetii isolates from Portugal based on MST and MLVA typing. Santos AS, Tilburg JJ, Botelho A, Barahona MJ, Núncio MS, Nabuurs-Franssen MH, Klaassen CH. SourceCentre for Vectors and Infectious Diseases Research Doutor Francisco Cambournac, National Institute of Health Dr. Ricardo Jorge (CEVDI/INSA), Águas de Moura, Portugal. Electronic address: ana.santos@insa.min-saude.pt. Abstract The temporal and spatial diversity of Coxiella burnetii genotypes associated with human and animal disease in Portugal was analysed using a 6-locus multiple-locus variable-number tandem repeat analysis (MLVA) and a 10-locus multi-spacer sequence typing (MST) panel. Fifteen cultured C. burnetii isolates from 13 Q fever patients and a stillborn goat and 6 additional PCR-positive ruminant tissue samples obtained during 2006-2011 were included in this study. Seven MLVA genotypes (types S-Y) were obtained, including 4 new MLVA types (U, V, W, and X), all corresponding to 3 MST profiles (types 4, 8, and 13) previously reported from France and Spain. MLVA types U-Y, all belonging to MST type 4, were found in acute Q fever patients from the districts of Évora, Faro, Lisbon, and Setúbal. Different MLVA types were associated with goats from Castelo Branco district (S) and chronic Q fever patients from both Castelo Branco and Lisboa districts (S and T), matching with MST types 13 and 8, respectively. In conclusion, a genotypic diversity of C. burnetii consistent with a non-outbreak situation was identified. The involvement of different genotypes in acute and chronic Q fever was found, linking one of the chronic genotypes to goats from the eastern region of the country.por
dc.identifier.citationInt J Med Microbiol. 2012 Nov;302(6):253-6. Epub 2012 Oct 4por
dc.identifier.issn1438-4221
dc.identifier.otherdoi: 10.1016/j.ijmm.2012.08.003
dc.identifier.urihttp://hdl.handle.net/10400.18/1090
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherUrban and Fischer / Elsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1438422112000549
dc.subjectCoxiella burnetiipor
dc.subjectHumanpor
dc.subjectAnimalpor
dc.subjectMLVApor
dc.subjectMSTpor
dc.subjectPortugalpor
dc.subjectInfecções Sistémicas e Zoonosespor
dc.titleGenotypic diversity of clinical Coxiella burnetii isolates from Portugal based on MST and MLVA-typingpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage256por
oaire.citation.startPage253por
oaire.citation.titleInternational Journal of Medical Microbiologypor
oaire.citation.volume302por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

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