Logo do repositório
 
Publicação

Proteomic and metabolomic profiling of methicillin-resistant Staphylococcus aureus associated with invasive vs. non-invasive infections: uncovering key biomarkers and pathogenic pathways

2026-05-06Artigo científico Acesso aberto
Ver registo simples
datacite.subject.fosCiências Naturais::Ciências Biológicas
dc.contributor.authorBoucherabine, Syrine
dc.contributor.authorGiddey, Alexander D.
dc.contributor.authorNassar, Rania
dc.contributor.authorMohamed, Lobna
dc.contributor.authorVerma, Subham
dc.contributor.authorSoares, Nelson C.
dc.contributor.authorSenok, Abiola
dc.date.accessioned2026-10-01T13:10:21Z
dc.date.available2026-10-01T13:10:21Z
dc.date.issued2026-05-06
dc.description.abstractIntroduction: Understanding the behavioral differences between invasive and non-invasive methicillin-resistant Staphylococcus aureus (MRSA) is essential for unraveling infection mechanisms and identifying biomarkers with translational potential. This study compared the proteomic and metabolomic profiles of MRSA isolates from diverse clinical presentations to uncover distinct molecular signatures. Methods: Invasive isolates were obtained from blood cultures (n = 23), while non-invasive isolates were derived from superficial skin infections (n = 49) and nasal colonizers (n = 24) from screening swabs. Proteins and metabolites were simultaneously extracted using a dual-phase methanol-based protocol. Proteomic analysis was performed on the Orbitrap Exploris 480, while metabolites were characterized using a TimsTOF mass spectrometer with an Apollo II electrospray ionization source. Data-independent acquisition (DIA) was applied, with peptide assignment carried out in DIA-NN and metabolite analysis using MetaboScape® 4.0. Results: Across all isolates, 2,000 proteins and 150 metabolites were identified. Comparative analysis revealed that invasive isolates exhibited consistently higher levels of two metabolites (sphinganine and phosphoserine) and one protein (staphylococcal secretory antigen SsaA2) compared to non-invasive. In contrast, three metabolites (cytidine, benzoic acid, and guanosine) and two proteins (small ribosomal subunit protein bS20 and bifunctional autolysin) were significantly reduced in invasive isolates. These findings highlight key molecular differences underpinning invasive potential in MRSA, providing insights into candidate diagnostic and therapeutic biomarkers. These findings highlight critical biological differences between invasive and non-invasive MRSA, offering valuable insights into potential diagnostic and therapeutic biomarkers.eng
dc.description.sponsorship((The project was funded by Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, UAE internal research grant (Ref#: MBRU-CM-RG2022-01). This study was supported by the Al Jalila Foundation.
dc.identifier.citationFront Microbiol. 2026 May 6:17:1798070. doi: 10.3389/fmicb.2026.1798070. eCollection 2026
dc.identifier.doi10.3389/fmicb.2026.1798070
dc.identifier.eissn1664-302X
dc.identifier.pmid42169777
dc.identifier.urihttp://hdl.handle.net/10400.18/11364
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media
dc.relation.hasversionhttps://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2026.1798070/full
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectMRSA
dc.subjectInvasive
dc.subjectMetabolomic
dc.subjectMethicillin Resistant Staphylococcus aureus
dc.subjectNon-invasive
dc.subjectProteomics
dc.subjectGenómica Funcional e Estrutural
dc.titleProteomic and metabolomic profiling of methicillin-resistant Staphylococcus aureus associated with invasive vs. non-invasive infections: uncovering key biomarkers and pathogenic pathwayseng
dc.typejournal article
dcterms.referenceshttps://public-pages-files-2025.frontiersin.org/articles/1798070/file/Supplementary_file_1.docx/1798070_supplementary-file_1/1
dcterms.referenceshttps://public-pages-files-2025.frontiersin.org/articles/1798070/file/Table_1.docx/1798070_table_1/1
dspace.entity.typePublication
oaire.citation.startPage1798070
oaire.citation.titleFrontiers in Microbiology
oaire.citation.volume17
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
2026, Boucherabine, Proteomic and metabolomic profiling of methicillin resistant Staphylococcus aureus.pdf
Tamanho:
1.68 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
4.03 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

DGH - Artigos em revistas internacionais
INSA - Artigos em revistas internacionais