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Toxicological impact of acute exposure to E171 food additive and TiO2 nanoparticles on a co-culture of Caco-2 and HT29-MTX intestinal cells

2018-11-22Journal article Restricted access
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dc.contributor.authorDorier, Marie
dc.contributor.authorTisseyre, Céline
dc.contributor.authorDussert, Fanny
dc.contributor.authorBéal, David
dc.contributor.authorArnal, Marie-Edith
dc.contributor.authorDouki, Thierry
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorLaffon, Blanca
dc.contributor.authorFraga, Sónia
dc.contributor.authorBrandão, Fátima
dc.contributor.authorHerlin-Boime, Nathalie
dc.contributor.authorBarreau, Frédérick
dc.contributor.authorRabilloud, Thierry
dc.contributor.authorCarriere, Marie
dc.date.accessioned2019-03-06T15:55:19Z
dc.date.available2019-03-06T15:55:19Z
dc.date.issued2018-11-22
dc.description.abstractTiO2 particles are widely used in products for everyday consumption, such as cosmetics and food; their possible adverse effects on human health must therefore be investigated. The aim of this study was to document in vitro impact of the food additive E171, i.e. TiO2, and of TiO2 nanoparticles, on a co-culture of Caco-2 and HT29-MTX cells, which is an in vitro model for human intestine. Cells were exposed to TiO2 particles three days after seeding, i.e. while they were not fully differentiated. Cell viability, reactive oxygen species (ROS) levels and DNA integrity were assessed, by MTT assay, DCFH-DA assay, alkaline and Fpg-modified comet assay and 8-oxo-dGuo measurement by HPLC-MS/MS. The mRNA expression of genes involved in ROS regulation, DNA repair via base-excision repair, and endoplasmic reticulum stress was assessed by RT-qPCR. Exposure to TiO2 particles resulted in increased intracellular ROS levels, but did not impair cell viability and did not cause any oxidative damage to DNA. Only minor changes in mRNA expression were detected. Altogether, this shows that E171 food additive and TiO2 nanoparticles only produce minor effects to this in vitro intestinal cell model.pt_PT
dc.description.sponsorshipThis work was supported by Atomic Energy and Alternative Energies Commission (CEA) through the ‘Toxicology’ research program (IMAGINATOX grant); the French Environment and Energy Management Agency (ADEME); the French Agency for Food, Environmental and Occupational Health & Safety (ANSES) within the framework of the National Research Program for Environmental and Occupational Health (PNR-EST, EST-2013/1/024, NanoGut grant) and the French National Research Agency (ANR, ANR-16-CE34-0011-01, PAIPITO grant). It is a contribution to the Labex Serenade (ANR-11- LABX-0064) funded by the French Government’s “Investissements d’Avenir” ANR program, through the A*MIDEX project (ANR-11-IDEX0001-02). It has been performed in the frame of the hCOMET COST action (CA15132). The authors would like to thank T. Lesuffleur (INSERM U938, Paris, France) for generous gift of HT29-MTX cells.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMutat Res Gen Tox En. 2018 Nov 22:1-8. doi: 10.1016/j.mrgentox.2018.11.004pt_PT
dc.identifier.doi10.1016/j.mrgentox.2018.11.004pt_PT
dc.identifier.issn1383-5718
dc.identifier.urihttp://hdl.handle.net/10400.18/6076
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1383571818302493pt_PT
dc.subjectNanoparticlept_PT
dc.subjectTiO2 E171pt_PT
dc.subjectFood Additivept_PT
dc.subjectToxicitypt_PT
dc.subjectIntestinept_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleToxicological impact of acute exposure to E171 food additive and TiO2 nanoparticles on a co-culture of Caco-2 and HT29-MTX intestinal cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage8pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleMutation Research - Genetic Toxicology and Environmental Mutagenesispt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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