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Coxiella burnetii DNA detected in domestic ruminants and wildlife from Portugal

2015-10-22Journal article Restricted access
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dc.contributor.authorCumbassá, A.
dc.contributor.authorBarahona, M.J.
dc.contributor.authorCunha, M.V.
dc.contributor.authorAzorín, B.
dc.contributor.authorFonseca, C.
dc.contributor.authorRosalino, L.M.
dc.contributor.authorTilburg, J.
dc.contributor.authorHagen, F.
dc.contributor.authorSantos, A.S.
dc.contributor.authorBotelho, A.
dc.date.accessioned2017-02-21T10:39:29Z
dc.date.available2019-07-29T00:30:08Z
dc.date.issued2015-10-22
dc.description.abstractCoxiella burnetii is the etiological agent of Q fever or Coxiellosis, a zoonosis mainly affecting domestic ruminants. Information on the population structure and epidemiology of C. burnetii in animals is scarce in Portugal. Evidence of C. burnetti infection was sought in domestic, wild and captive animals based on the detection of bacterial DNA. Tissue samples from 152 domestic animals (cattle = 24, goats = 51, sheep = 76 and swine = 1), 55 wild carnivores (Egyptian mongoose = 45, red fox = 4, common genet = 3, weasel = 2 and European badger = 1) and 22 zoo animals (antelopes = 15, impala = 1; rhinoceros = 1, deer = 2, zebras = 2 and giraffe = 1) were screened by nested-touchdown PCR. Cloacae swabs from 19 griffon vultures were also analysed. Among the domestic ruminants, goats presented the highest prevalence of infection (23.53%), followed by cattle, (20.83%) and sheep (10.53%). C. burnetii DNA was also detected in five Egyptian mongooses and two antelopes and one giraffe. Using a 6-locus multiple-locus variable-number tandem repeat analysis (MLVA-6) six complete genotypes, T, I and CM and the first reported CN, CO and CP, were identified, respectively, in small ruminants and Egyptian mongooses. Clustering analysis of genotypes exposed four distinct groups, according to detection source, enlightening an apparent association between C. burnetii genotype and host.pt_PT
dc.description.sponsorshipThis work was funded by the project PTDC/SAU-SAP/115266/2009 from Fundação para a Ciência e a Tecnologia (FCT). Postdoctoral fellowship (SFRH/BPD/35842/2007) was funded by FCT and Fundo Social Europeu (III Quadro Comunitário de Apoio).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVet Microbiol. 2015 Oct 22;180(1-2):136-41. doi: 10.1016/j.vetmic.2015.07.030. Epub 2015 Jul 28pt_PT
dc.identifier.doi10.1016/j.vetmic.2015.07.030pt_PT
dc.identifier.issn0378-1135
dc.identifier.urihttp://hdl.handle.net/10400.18/4296
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationQ fever - from diagnosis to eco-epidemiological investigation of Coxiella burnetii in the context of human infection
dc.relation.publisherversionhttp://www.sciencedirect.com/science/journal/03781135pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectQ Feverpt_PT
dc.subjectCoxiella Burnetiipt_PT
dc.subjectReproductive Disorderspt_PT
dc.subjectZoonosispt_PT
dc.subjectMLVA-6 Typingpt_PT
dc.subjectWild Carnivorespt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.subjectPortugal
dc.titleCoxiella burnetii DNA detected in domestic ruminants and wildlife from Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleQ fever - from diagnosis to eco-epidemiological investigation of Coxiella burnetii in the context of human infection
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-SAP%2F115266%2F2009/PT
oaire.citation.endPage141pt_PT
oaire.citation.startPage136pt_PT
oaire.citation.titleVeterinary Microbiologypt_PT
oaire.citation.volume180(1-2)pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication855b2602-1f42-4d60-ab76-44210d556ab6
relation.isProjectOfPublication.latestForDiscovery855b2602-1f42-4d60-ab76-44210d556ab6

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