Publication
Characterization of the inhibitor-resistant SHV β-lactamase SHV-107 in a clinical Klebsiella pneumoniae strain co-producing GES-7 enzyme
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Ferreira, Eugénia | |
| dc.contributor.author | Cougnoux, Antony | |
| dc.contributor.author | Albuquerque, Luís | |
| dc.contributor.author | Caniça, Manuela | |
| dc.contributor.author | Bonnet, Richard | |
| dc.date.accessioned | 2013-02-14T17:04:47Z | |
| dc.date.available | 2013-02-14T17:04:47Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | The clinical Klebsiella pneumoniae INSRA6884 strain exhibited nonsusceptibility to all penicillins tested (MICs of 64 to>2,048 g/ml). The MICs of penicillins were weakly reduced by clavulanate (from 2,048 to 512 g/ml), and tazobactam restored piperacillin susceptibility. Molecular characterization identified the genes blaGES-7 and a new -lactamase gene, blaSHV-107, which encoded an enzyme that differed from SHV-1 by the amino acid substitutions Leu35Gln and Thr235Ala. The SHV-107-producing Escherichia coli strain exhibited only a -lactam resistance phenotype with respect to amoxicillin, ticarcillin, and amoxicillinclavulanate combination. The kinetic parameters of the purified SHV-107 enzyme revealed a high affinity for penicillins. However, catalytic efficiency for these antibiotics was lower for SHV-107 than for SHV-1. No hydrolysis was detected against oxyimino- -lactams. The 50% inhibitory concentration (IC50) for clavulanic acid was 9-fold higher for SHV-107 than for SHV-1, but the inhibitory effects of tazobactam were unchanged. Molecular dynamics simulation suggested that the Thr235Ala substitution affects the accommodation of clavulanate in the binding site and therefore its inhibitory activity. | por |
| dc.description.sponsorship | This work was supported financially by the project POCTI/ESP/43037 from Fundação para a Ciência e a Tecnologia, Lisbon, Portugal, awarded to M. Caniça, and by a grant from INRA and Ministère de l’Education Nationale, de l’Enseignement Supérieur et de la Recherche (Paris, France), awarded to R. Bonnet. V. Manageiro was supported by grant SFRH/BD/32578/2006 from Fundação para a Ciência e a Tecnologia, Lisbon,Portugal. | por |
| dc.identifier.citation | Antimicrob Agents Chemother. 2012 Feb;56(2):1042-6. Epub 2011 Nov 14. | por |
| dc.identifier.issn | 0066-4804 | |
| dc.identifier.other | doi:10.1128/AAC.01444-10 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/1381 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | American Society for Microbiology | por |
| dc.relation.publisherversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264250/ | por |
| dc.subject | Antibiotic Resistance | por |
| dc.subject | β-lactam Inhibitor | por |
| dc.subject | β-lactamase | por |
| dc.subject | ESBL | por |
| dc.subject | Inhibitor-resistant SHV | por |
| dc.subject | Resistência aos Antimicrobianos | por |
| dc.title | Characterization of the inhibitor-resistant SHV β-lactamase SHV-107 in a clinical Klebsiella pneumoniae strain co-producing GES-7 enzyme | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1046 | por |
| oaire.citation.startPage | 1042 | por |
| oaire.citation.title | Antimicrobial Agents and Chemotherapy | por |
| oaire.citation.volume | 56(2) | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |