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Serum proteomics signature of Cystic Fibrosis patients: A complementary 2-DE and LC–MS/MS approach

2011-01-01Journal article Restricted access
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dc.contributor.authorCharro, Nuno
dc.contributor.authorHood, Brian L.
dc.contributor.authorFaria, Daniel
dc.contributor.authorPacheco, Paula
dc.contributor.authorAzevedo, Pilar
dc.contributor.authorLopes, Carlos
dc.contributor.authorBugalho de Almeida, António
dc.contributor.authorCouto, Francisco M.
dc.contributor.authorConrads, Thomas P.
dc.contributor.authorPenque, Deborah
dc.date.accessioned2011-09-07T14:13:33Z
dc.date.available2011-09-07T14:13:33Z
dc.date.issued2011-01-01
dc.description.abstractComplementary 2D-PAGE and ‘shotgun’ LC–MS/MS approaches were combined to identify medium and low-abundant proteins in sera of Cystic Fibrosis (CF) patients (mild or severe pulmonary disease) in comparison with healthy CF-carrier and non-CF carrier individuals aiming to gain deeper insights into the pathogenesis of this multifactorial genetic disease. 78 differentially expressed spots were identified from 2D-PAGE proteome profiling yielding 28 identifications and postulating the existence of post-translation modifications (PTM). The ‘shotgun’ approach highlighted altered levels of proteins actively involved in CF: abnormal tissue/airway remodeling, protease/antiprotease imbalance, innate immune dysfunction, chronic inflammation, nutritional imbalance and Pseudomonas aeruginosa colonization. Members of the apolipoproteins family (VDBP, ApoA-I, and ApoB) presented gradually lower expression from non-CF to CF-carrier individuals and from those to CF patients, results validated by an independent assay. The multifunctional enzyme NDKB was identified only in the CF group and independently validated by WB. Its functions account for ion sensor in epithelial cells, pancreatic secretion, neutrophil-mediated inflammation and energy production, highlighting its physiological significance in the context of CF. Complementary proteomics-based approaches are reliable tools to reveal pathways and circulating proteins actively involved in a heterogeneous disease such as CF.por
dc.identifier.citationJ Proteomics. 2011 Jan 1;74(1):110-26. Epub 2010 Oct 13por
dc.identifier.issn1874-3919
dc.identifier.otherdoi:10.1016/j.jprot.2010.10.001
dc.identifier.urihttp://hdl.handle.net/10400.18/125
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationProteomics of Chronic Lung Diseases Leading to Biomarkers and Therapeutic Target Discovery
dc.relation.urihttp://www.sciencedirect.com/science/article/pii/S1874391910002897por
dc.subjectCystic Fibrosispor
dc.subjectSerum proteome profilingpor
dc.subject2DE-MALDI-TOF/TOF MSpor
dc.subjectShotgun LC–MS/MSpor
dc.subjectLabel-free proteomicspor
dc.subjectGenómica Funcional e Estruturalpor
dc.titleSerum proteomics signature of Cystic Fibrosis patients: A complementary 2-DE and LC–MS/MS approachpor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleProteomics of Chronic Lung Diseases Leading to Biomarkers and Therapeutic Target Discovery
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FSAU-MMO%2F56163%2F2004/PT
oaire.citation.endPage126por
oaire.citation.startPage110por
oaire.citation.titleJournal of Proteomicspor
oaire.fundingStreamPOCI
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isProjectOfPublicationbe2672ab-2bb0-44ef-84dd-60adb43dbf00
relation.isProjectOfPublication.latestForDiscoverybe2672ab-2bb0-44ef-84dd-60adb43dbf00

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