Publication
Molecular characterization of the HA gene of influenza type B viruses
| dc.contributor.author | Pechirra, Pedro | |
| dc.contributor.author | Nunes, Baltazar | |
| dc.contributor.author | Coelho, Anabela | |
| dc.contributor.author | Ribeiro, Carlos | |
| dc.contributor.author | Gonçalves, Paulo | |
| dc.contributor.author | Pedro, Sónia | |
| dc.contributor.author | Canto e Castro, Luísa | |
| dc.contributor.author | Rebelo-de-Andrade, Helena | |
| dc.date.accessioned | 2012-01-18T15:08:08Z | |
| dc.date.available | 2012-01-18T15:08:08Z | |
| dc.date.issued | 2005-12 | |
| dc.description.abstract | Abstract Nucleotide sequences of the HA1 subunit of influenza B viruses isolated in Portugal between 1994 and 2003 influenza winter seasons were analyzed by the Neighbor-Joining algorithm and rates of HA1 evolution estimated by linear regression. From 1994 to 2002, all influenza B viruses studied were of the Yamagata lineage. Strains isolated from 1994 to 1996, 1996 to 1999, and 1999 to 2002 revealed a high similarity with B/Beijing/184/93, B/Yamanashi/166/98, and B/Sichuan/379/99, respectively, and strains isolated during 1994-1995, 1996-1997, and 1998-1999 clustered in more than one branch of the phylogenetic tree. Victoria-related strains reappeared during 2002/2003 and formed only one branch in the phylogenetic tree revealing a closer relationship to B/Shandong/7/97. Evolutionary rates for strains from the Yamagata lineage were estimated as 3.82x10(-3) nucleotides/site/year and 2.62x10(-3) nucleotides/site/year for Victoria-related strains. In order to identify putative influenza B HA1 codons under selective pressure, a codon-substitution model for heterogeneous selective pressure at amino acid sites was used. A percentage of 97.3% of codons under negative selective pressure and 2.7% of codons under positive selective pressure (omega=dN/dS=2.65) were estimated, with posterior probability higher than 0.90. Amino acid sites 75, 197, and 199 were found more likely to be under positive selective pressure. | por |
| dc.identifier.citation | Pechirra P, Nunes B, Coelho A, Ribeiro C, Gonçalves P, Pedro S, Castro LC, Rebelo-de-Andrade H. 2005.Molecular characterization of the HA gene of influenza type B viruses. J Med Virol. 2005 Dec;77(4):541-9 | por |
| dc.identifier.issn | 0146-6615 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/397 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Wiley-Blackwell - Wiley-Liss | por |
| dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/jmv.20490/pdf | por |
| dc.subject | Infecções Respiratórias | por |
| dc.subject | Influenza | por |
| dc.subject | Phylogeny | por |
| dc.subject | Genetic analysis | por |
| dc.subject | Selective pressure | por |
| dc.subject | Adaptive evolution | por |
| dc.subject | Estados de Saúde e de Doença | por |
| dc.title | Molecular characterization of the HA gene of influenza type B viruses | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 549 | por |
| oaire.citation.startPage | 541 | por |
| oaire.citation.title | Journal of Medical Virology | por |
| rcaap.rights | restrictedAccess | por |
| rcaap.type | article | por |