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Assessing the antibiotic susceptibility of freshwater Cyanobacteria spp.

2015-08-11Journal article Open access
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dc.contributor.authorDias, Elsa
dc.contributor.authorOliveira, Micaela
dc.contributor.authorJones-Dias, Daniela
dc.contributor.authorVasconcelos, Vitor
dc.contributor.authorFerreira, Eugénia
dc.contributor.authorManageiro, Vera
dc.contributor.authorCaniça, Manuela
dc.date.accessioned2016-03-01T17:04:02Z
dc.date.available2016-03-01T17:04:02Z
dc.date.issued2015-08-11
dc.description.abstractFreshwater is a vehicle for the emergence and dissemination of Antibiotic resistance. Cyanobacteria are ubiquitous in freshwater, where they are exposed to antibiotics and resistant organisms, but their role on water resistome was never evaluated. Data concerning the effects of antibiotics on cyanobacteria, obtained by distinct methodologies, is often contradictory. This emphasizes the importance of developing procedures to understand the trends of antibiotic susceptibility in cyanobacteria. In this study we aimed to evaluate the susceptibility of four cyanobacterial isolates from different genera (Microcystis aeruginosa, Aphanizomenon gracile, Chrisosporum bergii, Planktothix agradhii), and among them nine isolates from the same specie (M.aeruginosa) to distinct antibiotics (amoxicillin, ceftazidime, ceftriaxone, kanamycine, gentamicine, tetracycline, trimethoprim, nalidixicacid, norfloxacin). We used a method adapted from the bacteria standard broth microdilution. Cyanobacteria were exposed to serial dilution of each antibiotic (0.0015–1.6mg/L) in Z8 medium (20±1◦C; 14/10hL/D cycle; light intensity 16±421μEm−s−). Cell growth was followed overtime (OD450nm/microscopic examination) and the minimum inhibitory concentrations (MICs) were calculated for each antibiotic/ isolate. We found that β-lactams exhibited the lower MICs, aminoglycosides, tetracycline and norfloxacine presented intermediate MICs; none of the isolates were susceptible to trimethoprim and nalidixic acid. The reduced susceptibility of all tested cyanobacteria to some antibiotics suggests that they might be naturally non-susceptible to these compounds, or that they might became non-susceptible due to antibiotic contamination pressure, or to the transfer of genes from resistant bacteria present in the environment.pt_PT
dc.description.sponsorshipED, VM, and DJ have received research funding from Fundação para a Ciência e Tecnologia via grants SFRH/BPD/77981/2011, SFRH/BPD/77486/2011, and SFRH/BD/80001/2011, respectively. The authors thank Fundação para a Ciência e a Tecnologia (FCT) for project grant PEst-OE/AGR/UI0211/2011-2014, Strategic Project UI211-2011-2014.pt_PT
dc.identifier.citationFront Microbiol. 2015 Aug 11;6:799. doi: 10.3389/fmicb.2015.00799. eCollection 2015pt_PT
dc.identifier.doi10.3389/fmicb.2015.00799pt_PT
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.18/3557
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relation.publisherversionhttp://journal.frontiersin.org/article/10.3389/fmicb.2015.00799/fullpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCyanobacteriapt_PT
dc.subjectAntibioticspt_PT
dc.subjectMinimum Inhibitory Concentrationspt_PT
dc.subjectReduced Susceptibilitypt_PT
dc.subjectWaterpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.subjectÁgua e Solopt_PT
dc.titleAssessing the antibiotic susceptibility of freshwater Cyanobacteria spp.pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/PEst-OE%2FAGR%2FUI0211%2F2014/PT
oaire.citation.endPage799
oaire.citation.startPage799pt_PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume6pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationd3e4188b-642e-4ad7-9a4d-aeea65a89449
relation.isProjectOfPublication.latestForDiscoveryd3e4188b-642e-4ad7-9a4d-aeea65a89449

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