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Effects of Zinc Oxide Nanoparticle Exposure on Human Glial Cells and Zebrafish Embryos

2023-08-01Journal article Open access
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dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorAlba-González, Anabel
dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorTouzani, Assia
dc.contributor.authorRamos-Pan, Lucía
dc.contributor.authorReis, Ana Teresa
dc.contributor.authorMoreda-Piñeiro, Jorge
dc.contributor.authorYáñez, Julián
dc.contributor.authorLaffon, Blanca
dc.contributor.authorFolgueira, Mónica
dc.date.accessioned2024-01-31T14:34:01Z
dc.date.available2024-01-31T14:34:01Z
dc.date.issued2023-08-01
dc.description(This article belongs to the Special Issue Antimicrobial Activity of Metal- and Metal-Oxide-Based Nanoparticles)pt_PT
dc.description.abstractZinc oxide nanoparticles (ZnO NPs) are among the most widely used nanomaterials. They have multiple applications in cosmetics, textiles, paints, electronics and, recently, also in biomedicine. This extensive use of ZnO NPs notably increases the probability that both humans and wildlife are subjected to undesirable effects. Despite being among the most studied NPs from a toxicological point of view, much remains unknown about their ecotoxicological effects or how they may affect specific cell types, such as cells of the central nervous system. The main objective of this work was to investigate the effects of ZnO NPs on human glial cells and zebrafish embryo development and to explore the role of the released Zn2+ ions in these effects. The effects on cell viability on human A172 glial cells were assessed with an MTT assay and morphological analysis. The potential acute and developmental toxicity was assessed employing zebrafish (Danio rerio) embryos. To determine the role of Zn2+ ions in the in vitro and in vivo observed effects, we measured their release from ZnO NPs with flame atomic absorption spectrometry. Then, cells and zebrafish embryos were treated with a water-soluble salt (zinc sulfate) at concentrations that equal the number of Zn2+ ions released by the tested concentrations of ZnO NPs. Exposure to ZnO NPs induced morphological alterations and a significant decrease in cell viability depending on the concentration and duration of treatment, even after removing the overestimation due to NP interference. Although there were no signs of acute toxicity in zebrafish embryos, a decrease in hatching was detected after exposure to the highest ZnO NP concentrations tested. The ability of ZnO NPs to release Zn2+ ions into the medium in a concentration-dependent manner was confirmed. Zn2+ ions did not seem entirely responsible for the effects observed in the glial cells, but they were likely responsible for the decrease in zebrafish hatching rate. The results obtained in this work contribute to the knowledge of the toxicological potential of ZnO NPs.pt_PT
dc.description.sponsorshipThis research was funded by the Ministry of Science and Innovation: MCIN/AEI/10.13039/501100011033 (grant PID2020-114908GA-I00), Xunta de Galicia (ED431B 2022/16 and ED481A 2019/003 to A.A-G.), CICA-Disrupting Project 2021SEM-B2, and Ministry of Education, Culture and Sport (BEAGAL18/00142 to V.V.).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Mol Sci. 2023 Aug 1;24(15):12297. doi: 10.3390/ijms241512297pt_PT
dc.identifier.doi10.3390/ijms241512297pt_PT
dc.identifier.issn1661-6596
dc.identifier.urihttp://hdl.handle.net/10400.18/9008
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/24/15/12297pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectZinc Oxide Nanoparticles (ZnO NPs)pt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectHuman A172 Glial Cellspt_PT
dc.subjectZebrafishpt_PT
dc.subjectZinc Oxide Nanoparticlespt_PT
dc.subjectZn2 + ionspt_PT
dc.subjectToxicologia
dc.titleEffects of Zinc Oxide Nanoparticle Exposure on Human Glial Cells and Zebrafish Embryospt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue15pt_PT
oaire.citation.startPage12297pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume24pt_PT
person.familyNameLemos Pereira Saúde Reis
person.givenNameAna Teresa
person.identifier.ciencia-id5E14-031E-EAE3
person.identifier.orcid0000-0001-7150-997X
rcaap.embargofctAcesso de acordo com a política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb735213d-ccf3-4f45-ad24-db085e06f38b
relation.isAuthorOfPublication.latestForDiscoveryb735213d-ccf3-4f45-ad24-db085e06f38b

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