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Publication
Bacteroides spp. and traditional fecal indicator bacteria in water quality assessment – An integrated approach for hydric resources management in urban centers
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Advisor(s)
Abstract(s)
As part of a sustainable water resources management, the Lisbon municipality identified groundwater and treated wastewater use increase as two opportunities for better and sustainable water use, with natural safeguard for public health as a priority. In this context, the aim of our research was to assess the suitability of the human-associated marker gene Bacteroides HF183 and the cattle feces-associated CowM2, in routine water quality monitoring as indicators for water use and reuse, providing a tool to more accurately assess public health risks. To this intent, Real-Time quantitative PCR was used for detection of human-associated marker gene Bacteroides HF183 and the bovine-associated CowM2, in a total of 67 samples - groundwater and wastewater at three different treatment stages of a Waste Water Treatment Plant, in Lisbon. HF183 marker gene was detected in treated and untreated wastewater samples, with significant concentration reductions from untreated (6,07 E+07 copies/mL) to secondary treated effluent (1,86 E+05 copies/mL) and a further decrease in tertiary treatment (5,74 E+04 copies/mL). In groundwater samples, this marker was also detected in concentrations ranging from 2,63 E+02 copies/mL to 2,24 E+03 copies/mL. CowM2 marker gene on the other hand was only detected in wastewater samples, with concentrations ranging from 2,47 E+02 copies/mL to 1,17 E+04 copies/mL. Our research indicates that the use of Bacteroides spp. in association with traditional fecal indicator bacteria (FIB) is advantageous for water managing entities in urban settings, such as Lisbon, were drainage system failures may occur. An integrated approach thus provides crucial and more adequate information towards mitigation and correction measures when fecal contamination is detected in environmental waters.
Highlights: Bacteroides were evaluated in an integrated hydric resources management approach; Mutual detection of Bacteroides and FIB is advantageous for water managing entities; HF183 marker was detected in groundwater, untreated and treated wastewater samples; Identifying the origin of groundwater contamination allows correction measures; HF183 marker may have a future role in evaluation of wastewater treatment.
Highlights: Bacteroides were evaluated in an integrated hydric resources management approach; Mutual detection of Bacteroides and FIB is advantageous for water managing entities; HF183 marker was detected in groundwater, untreated and treated wastewater samples; Identifying the origin of groundwater contamination allows correction measures; HF183 marker may have a future role in evaluation of wastewater treatment.
Description
Keywords
Bacteroides HF183 CowM2 Groundwater Water Reuse Water Quality Água e Solo Determinantes da Saúde e da Doença
Pedagogical Context
Citation
J Environ Manage. 2020 Oct 1;271:110989. doi: 10.1016/j.jenvman.2020.110989. Epub 2020 Jun 24
Publisher
Elsevier/ Academic Press