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Molecular epidemiology of invasive Haemophilus influenzae disease in Portugal: an update of the post-vaccine period, 2011-2018

2020-03-14Journal article Restricted access
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dc.contributor.authorHeliodoro, Catarina Isabel Moreira
dc.contributor.authorBettencourt, Célia Rodrigues
dc.contributor.authorBajanca-Lavado, Maria Paula
dc.contributor.authorPortuguese Group for the Study of Haemophilus influenzae invasive infection
dc.date.accessioned2020-10-26T17:59:43Z
dc.date.available2021-03-14T01:30:14Z
dc.date.issued2020-03-14
dc.description.abstractHaemophilus influenzae reference laboratory from Portugal characterized the entire collection of 260 H. influenzae invasive isolates received between 2011 and 2018, with the purpose of updating the last published data (2002-2010). Capsular serotypes and antimicrobial susceptibility patterns were determined. The ftsI gene encoding the transpeptidase domain of PBP3 was sequenced for β-lactamase-negative ampicillin-resistant (BLNAR) isolates. Multilocus sequence typing (MLST) was performed to examine genetic relatedness among isolates. The majority of H. influenzae invasive isolates are nonencapsulated (NTHi-79.2%). Among encapsulated isolates (20.8%), the most characterized serotype was serotype b (13.5%), followed by serotype f (3.1%), serotype a (2.7%), and serotype e (1.5%). In contrast to NTHi that mainly affected the elderly (64.0%; ≥ 65 years old), most encapsulated isolates were characterized in preschool children (55.6%). Comparing the two periods, β-lactamase production increased from 10.4 to 13.5% (p = 0.032) and low-BLNAR (MIC ≥ 1 mg/L) isolates from 7.7 to 10.5% (p = 0.017). NTHi showed high genetic diversity (60.7%), in opposition to encapsulated isolates that were clonal within each serotype. Interestingly, ST103 and ST57 were the predominant STs among NTHi, with ST103 being associated with β-lactamase-producers and ST57 with non-β-lactamase-producers. In Portugal, susceptible and genetically diverse NTHi H. influenzae continues to be responsible for invasive disease, mainly in the elderly. Nevertheless, we are now concerned with Hib circulating in children we believe to have been vaccinated. Our data reiterates the need for continued surveillance, which will be useful in the development of public health prevention strategies.pt_PT
dc.description.sponsorshipThis work was supported by National Institute of Health, Lisbon, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEur J Clin Microbiol Infect Dis. 2020 Aug;39(8):1471-1480. doi: 10.1007/s10096-020-03865-0. Epub 2020 Mar 14pt_PT
dc.identifier.doi10.1007/s10096-020-03865-0pt_PT
dc.identifier.issn0934-9723
dc.identifier.urihttp://hdl.handle.net/10400.18/7212
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10096-020-03865-0pt_PT
dc.subjectHaemophilus influenzaept_PT
dc.subjectInvasive Diseasept_PT
dc.subjectSerotype bpt_PT
dc.subjectNon-encapsulatedpt_PT
dc.subjectMLSTpt_PT
dc.subjectHaemophilus influenzae Invasive infection in Portugalpt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.subjectPortugalpt_PT
dc.titleMolecular epidemiology of invasive Haemophilus influenzae disease in Portugal: an update of the post-vaccine period, 2011-2018pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1480pt_PT
oaire.citation.issue8pt_PT
oaire.citation.startPage1471pt_PT
oaire.citation.titleEuropean Journal of Clinical Microbiology and Infectious Diseasespt_PT
oaire.citation.volume39pt_PT
rcaap.embargofctDe acordo com a política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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