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Surveying genetic markers of antibiotic resistance and genomic background in Chlamydia trachomatis: insights from a multiplex NGS-based approach in clinical strains from Portugal

2025-04-02Journal article Restricted access
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dc.contributor.authorLodhia, Zohra
dc.contributor.authorda Silva, Jorge Costa
dc.contributor.authorCorreia, Cristina
dc.contributor.authorCordeiro, Dora
dc.contributor.authorJoão, Inês
dc.contributor.authorCarreira, Teresa
dc.contributor.authorSchäfer, Sandra
dc.contributor.authorAliyeva, Elzara
dc.contributor.authorPortugal, Clara
dc.contributor.authorMonge, Isabel
dc.contributor.authorGonçalves, Elsa
dc.contributor.authorMatos, Susana
dc.contributor.authorDias, Ana Paula
dc.contributor.authorCôrte-Real, Rita
dc.contributor.authorCarpinteiro, Dina
dc.contributor.authorDuarte, Sílvia
dc.contributor.authorVieira, Luís
dc.contributor.authorGomes, João Paulo
dc.contributor.authorBorges, Vítor
dc.contributor.authorBorrego, Maria José
dc.date.accessioned2025-11-25T16:48:48Z
dc.date.available2025-11-25T16:48:48Z
dc.date.issued2025-04-02
dc.description.abstractObjectives: To survey genetic markers of potential antimicrobial resistance (AMR) to macrolides and fluoroquinolones among Chlamydia trachomatis–positive samples from the collection of the Portuguese National Reference Laboratory for Sexually Transmitted Infections (STIs), and explore a multiplex PCR approach coupled with NGS to provide complementary information regarding a strain’s genomic backbone. Methods: A total of 502 C. trachomatis–positive samples, mostly anorectal exudates, were subjected to PCR and sequencing of five targets, including loci potentially driving AMR (23S rRNA, gyrA and parC) and loci potentially informative about a strain’s genomic backbone with emphasis on differentiation of lymphogranuloma venereum (LGV)/non-LGV and L2/L2b (a 9 bp insertion in pmpH, a 74 bp insertion upstream from CT105 and the polymorphic CT442). Results: No samples evidenced 23S rRNA mutations recognizably linked to macrolide resistance. Three samples harboured the Ser83Ile mutation in GyrA putatively driving fluoroquinolone resistance: two recombinant L2-L2b/D-Da (0.4%) and one L2 (0.2%). The screened regions in pmpH, upstream CT105 and CT442 were fully concordant with LGV/non-LGV differentiation. As expected, the pmpH L2b-specific genetic trait locus was detected in all L2b and recombinant L2-L2b/D-Da ompA genotypes, but also in 96.0% of L2 specimens, which also likely possess an L2b genomic backbone. The insertion upstream from CT105 exhibited full LGV specificity, constituting a promising target for the development of rapid LGV diagnostic assays. Conclusions: This study contributes to enhancing the knowledge of C. trachomatis molecular epidemiology, suggesting that the known genetic determinants of AMR are not disseminated in clinical C. trachomatis strains, and presents an exploratory approach that can be suitable for LGV/non-LGV and L2/L2b genomic background differentiation.eng
dc.identifier.citationJ Antimicrob Chemother. 2025 Apr 2;80(4):1072-1079. doi: 10.1093/jac/dkaf036
dc.identifier.doi10.1093/jac/dkaf036
dc.identifier.eissn1460-2091
dc.identifier.issn0305-7453
dc.identifier.pmid39960073
dc.identifier.urihttp://hdl.handle.net/10400.18/10626
dc.language.isoeng
dc.peerreviewedyes
dc.publisherOxford University Press
dc.relation.hasversionhttps://academic.oup.com/jac/article-abstract/80/4/1072/8017874?redirectedFrom=fulltext
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/
dc.subjectGenetic Markers
dc.subjectAntibiotic Resistance
dc.subjectChlamydia Trachomatis
dc.subjectGenotype
dc.subjectPortugal
dc.subjectInfecções Sexualmente Transmissíveis
dc.subjectResistência aos Antimicrobianos
dc.titleSurveying genetic markers of antibiotic resistance and genomic background in Chlamydia trachomatis: insights from a multiplex NGS-based approach in clinical strains from Portugaleng
dc.typejournal article
dcterms.referenceshttps://academic.oup.com/jac/article-abstract/80/4/1072/8017874?redirectedFrom=fulltext#supplementary-data
dspace.entity.typePublication
oaire.citation.endPage1079
oaire.citation.issue4
oaire.citation.startPage1072
oaire.citation.titleJournal of Antimicrobial Chemotherapy
oaire.citation.volume80
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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