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Reverse genetics vaccine seeds for influenza: Proof of concept in the source of PB1 as a determinant factor in virus growth and antigen yield

2016-09Journal article Restricted access
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dc.contributor.authorGíria, Marta
dc.contributor.authorSantos, Luís
dc.contributor.authorLouro, João
dc.contributor.authorRebelo de Andrade, Helena
dc.date.accessioned2017-03-07T16:21:44Z
dc.date.available2021-05-28T00:30:09Z
dc.date.issued2016-09
dc.description.abstractGrowth deficits of reverse genetics vaccine seeds have compromised effective immunization. The impairment has been attributed to sub-optimal protein interactions. Some level of dependence may exist between PB1 and antigenic glycoproteins, however, further research is necessary to clarify the extent to which it can be used in favor of seed production. Our objective was to establish proof of concept on the phenotypic outcome of PB1 source in the PR8: A(H1N1)pdm09 reassortants. Reassortants were generated with the gene constellation of the classical 6:2 PR8: HA, NApdm09 seed prototype and the 5:3 reassortant PR8: HA, NA, PB1pdm09. Viral growth and antigen yield were evaluated 12-60h post-infection. The 5:3 reassortant presented statistically significant growth and antigen yield improvements when compared to the 6:2. We believe these findings to be of promising value to vaccine research towards an improvement of reverse genetic seeds, an overall more cost-effective vaccine manufacture and timely delivery.pt_PT
dc.description.sponsorshipThis research has been funded by the Fundação para a Ciência e Tecnologia Grants PTDC/SAU-MIC/122780/2010, SFRH/BD/65211/2009, SFRH/BD/62676/2009 and SFRH/BD/48532/2008.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVirology. 2016 Sep;496:21-7. doi: 10.1016/j.virol.2016.05.015. Epub 2016 May 27pt_PT
dc.identifier.doi10.1016/j.virol.2016.05.015pt_PT
dc.identifier.issn0042-6822
dc.identifier.urihttp://hdl.handle.net/10400.18/4550
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ Academic Presspt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0042682216301246pt_PT
dc.subjectInfluenza Seed Virusespt_PT
dc.subjectReverse Geneticspt_PT
dc.subjectPolymerase Basic Protein 1pt_PT
dc.subjectGene Constellationpt_PT
dc.subjectVirus Growthpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleReverse genetics vaccine seeds for influenza: Proof of concept in the source of PB1 as a determinant factor in virus growth and antigen yieldpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-MIC%2F122780%2F2010/PT
oaire.citation.endPage27pt_PT
oaire.citation.startPage21pt_PT
oaire.citation.titleVirologypt_PT
oaire.citation.volume496pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication761e3b35-e365-41a4-a08a-905e99193b1b
relation.isProjectOfPublication.latestForDiscovery761e3b35-e365-41a4-a08a-905e99193b1b

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