Publication
Genomic Study of a Clostridium difficile Multidrug Resistant Outbreak-Related Clone Reveals Novel Determinants of Resistance
| dc.contributor.author | Isidro, Joana | |
| dc.contributor.author | Menezes, Juliana | |
| dc.contributor.author | Serrano, Mónica | |
| dc.contributor.author | Borges, Vítor | |
| dc.contributor.author | Paixão, Pedro | |
| dc.contributor.author | Mimoso, Margarida | |
| dc.contributor.author | Martins, Filomena | |
| dc.contributor.author | Toscano, Cristina | |
| dc.contributor.author | Santos, Andrea | |
| dc.contributor.author | Henriques, Adriano O. | |
| dc.contributor.author | Oleastro, Mónica | |
| dc.date.accessioned | 2019-03-21T15:40:19Z | |
| dc.date.available | 2019-03-21T15:40:19Z | |
| dc.date.issued | 2018-12-06 | |
| dc.description.abstract | Background: Clostridium difficile infection (CDI) is prevalent in healthcare settings. The emergence of hypervirulent and antibiotic resistant strains has led to an increase in CDI incidence and frequent outbreaks. While the main virulence factors are the TcdA and TcdB toxins, antibiotic resistance is thought to play a key role in the infection by and dissemination of C. difficile. Methods: A CDI outbreak involving 12 patients was detected in a tertiary care hospital, in Lisbon, which extended from January to July, with a peak in February, in 2016. The C. difficile isolates, obtained from anaerobic culture of stool samples, were subjected to antimicrobial susceptibility testing with Etest®strips against 11 antibiotics, determination of toxin genes profile, PCR-ribotyping, multilocus variable-number tandem-repeat analysis (MLVA) and whole genome sequencing (WGS). Results: Of the 12 CDI cases detected, 11 isolates from 11 patients were characterized. All isolates were tcdA -/tcdB + and belonged to ribotype 017, and showed high level resistance to clindamycin, erythromycin, gentamicin, imipenem, moxifloxacin, rifampicin and tetracycline. The isolates belonged to four genetically related MLVA types, with six isolates forming a clonal cluster. Three outbreak isolates, each from a different MLVA type, were selected for WGS. Bioinformatics analysis showed the presence of several antibiotic resistance determinants, including the Thr82Ile substitution in gyrA, conferring moxifloxacin resistance, the substitutions His502Asn and Arg505Lys in rpoB for rifampicin resistance, the tetM gene, associated with tetracycline resistance, and two genes encoding putative aminoglycoside-modifying enzymes, aadE and aac(6')-aph(2″). Furthermore, a not previously described 61.3 kb putative mobile element was identified, presenting a mosaic structure and containing the genes ermG, mefA/msrD and vat, associated with macrolide, lincosamide and streptogramins resistance. A substitution found in a class B penicillin-binding protein, Cys721Ser, is thought to contribute to imipenem resistance. Conclusion: We describe an epidemic, tcdA -/tcdB +, multidrug resistant clone of C. difficile from ribotype 017 associated with a hospital outbreak, providing further evidence that the lack of TcdA does not impair the infectious potential of these strains. We identified several determinants of antimicrobial resistance, including new ones located in mobile elements, highlighting the importance of horizontal gene transfer in the pathogenicity and epidemiological success of C. difficile. | pt_PT |
| dc.description.sponsorship | This work was supported by the National Institute of Health Dr. Ricardo Jorge (Grant No. 2016DDI1284). This work was also supported by Project LISBOA-01-0145-FEDER-007660 (“Microbiologia Molecular, Estrutural e Celular”) funded by FEDER funds through COMPETE2020 – “Programa Operacional Competitividade e Internacionalização” (POCI), through ONEIDA project (LISBOA-01-0145-FEDER-016417) co-funded by FEEI – “Fundos Europeus Estruturais e de Investimento” from “Programa Operacional Regional Lisboa 2020,” by national funds from FCT – “Fundação para a Ciência e a Tecnologia” and by program IF (IF/00268/2013/CP1173/CT0006) to MS. Capillary sequencing and WGS were performed at Unidade de Tecnologia e Inovação (Departamento de Genética Humana, Instituto Nacional de Saúde Doutor Ricardo Jorge, Lisbon, Portugal). | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Front Microbiol. 2018 Dec 6;9:2994. doi: 10.3389/fmicb.2018.02994. eCollection 2018 | pt_PT |
| dc.identifier.doi | 10.3389/fmicb.2018.02994 | pt_PT |
| dc.identifier.issn | 1664-302X | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/6268 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Frontiers Media | pt_PT |
| dc.relation.publisherversion | https://www.frontiersin.org/articles/10.3389/fmicb.2018.02994/full | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Clostridium difficile | pt_PT |
| dc.subject | Multidrug Resistance | pt_PT |
| dc.subject | Outbreak | pt_PT |
| dc.subject | RT017 | pt_PT |
| dc.subject | Infecções Gastrointestinais | pt_PT |
| dc.title | Genomic Study of a Clostridium difficile Multidrug Resistant Outbreak-Related Clone Reveals Novel Determinants of Resistance | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.startPage | 2994 | pt_PT |
| oaire.citation.title | Frontiers in Microbiology | pt_PT |
| oaire.citation.volume | 9 | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |