Carotenoid stability in fruits, vegetables and working standards – Effect of storage temperature and time

Dias, M. Graça; Camões, M. Filomena; Oliveira, Luisa

http://hdl.handle.net/10400.18/2334

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
18 - Carotenoid stability in fruits, vegetables and working standards – Effect of storage temperature and time.pdf		763.31 KB	Adobe PDF	Download

Send Feedback

Authors

Dias, M. Graça

Camões, M. Filomena

Oliveira, Luisa

Abstract(s)

The effects of freezing and storage temperature on the mass fraction of a- and b-carotene, b-cryptoxanthin, lutein, lycopene and zeaxanthin in minimally processed fresh food products, were evaluated after sample preparation, extraction and saponification (only when strictly necessary). Effects of freezing and long-term stability were studied at two temperatures, 20 and 70 C, using high performance liquid chromatography (reversed phase columns, UV–Vis diode array detector) at time points during storage; measurement uncertainty was included in the evaluation. Stability of working standard solutions was also examined. Freezing did not affect the carotenoid mass fraction under the conditions studied. Carotenoids in orange, cherry, peach, apple, and kale were stable (except a-carotene and zeaxanthin in peach) for 13, 9.7, 5.7, 2.5 and 7.5 months, respectively. For these food sample matrices, no significant difference between the freezing/storage at 20 and 70 C was observed. Standard solutions (0.05– 5 lg/mL) were stable for at least 6 months at 70 C, except lycopene which at 0.05 lg/mL was apparently stable only for six weeks.