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Assessment of Antibiotic Resistance Among Isolates of Klebsiella spp. and Raoultella spp. in Wildlife and Their Environment from Portugal: A Positive Epidemiologic Outcome

2025-01-20Artigo científico Acesso aberto
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dc.contributor.authorSabença, Carolina
dc.contributor.authorde la Rivière, Rani
dc.contributor.authorBarros, Paulo
dc.contributor.authorCabral, João Alexandre
dc.contributor.authorSargo, Roberto
dc.contributor.authorSousa, Luís
dc.contributor.authorDapkevicius, Maria de Lurdes Enes
dc.contributor.authorSilva, Filipe
dc.contributor.authorLopes, Filipa
dc.contributor.authorAbrantes, Ana Carolina
dc.contributor.authorVieira-Pinto, Madalena
dc.contributor.authorCaniça, Manuela
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorTorres, Carmen
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2026-02-05T14:43:14Z
dc.date.available2026-02-05T14:43:14Z
dc.date.issued2025-01-20
dc.description(This article belongs to the Collection New Insights into Bacterial Pathogenesis)
dc.description.abstractOne of the significant challenges facing modern medicine is the rising rate of antibiotic resistance, which impacts public health, animal health, and environmental preservation. Evaluating antibiotic resistance in wildlife and their environments is crucial, as it offers essential insights into the dynamics of resistance patterns and promotes strategies for monitoring, prevention, and intervention. and genera isolates were recovered from fecal samples of wild animals and environmental samples using media without antibiotic supplementation. Antibiograms were performed for 15 antibiotics to determine the phenotypic resistance profile in these isolates. Extended-spectrum β-lactamase (ESBL) production was tested by the double-disc synergy test, and one ESBL-producing isolate was screened by PCR and whole-genome sequencing. Biofilm production was analyzed using the microtiter plate method. A total of 23 spp. and 3 spp. isolates were obtained from 312 fecal samples from wild animals, 9 spp. and 4 spp. isolates were obtained from 18 river and stream water samples, and 4 spp. and 3 spp. isolates from 48 soil samples. Regarding antibiotic resistance, only one isolate of from soil samples was an ESBL-producer and showed resistance to six antibiotics. This isolate harbored multiple β-lactams genes (, , , and ), as well as genes of resistance to quinolones, sulfonamides, tetracycline, aminoglycosides, and chloramphenicol, and belonged to the lineage ST307. Most of the spp. and spp. isolates were biofilm producers (except for one isolate), and 45.6% were weak biofilm producers, with the remaining being moderate to strong biofilm producers. We can conclude that antibiotic resistance is not widespread in these environment-associated isolates, which is a positive epidemiological outcome. However, identifying a single ESBL- isolate should serve as a warning of potential hotspots of resistance emergence.eng
dc.description.sponsorshipThis work was supported by the projects UIDB/00772/2020 (Doi:10.54499/UIDB/00772/2020), UIDB/04033/2020 (Doi:10.54499/UIDB/04033/2020), LA/P/0059/2020, and LA/P/0126/2020 (Doi:10.54499/LA/P/0126/2020) funded by the Portuguese Foundation for Science and Technology (FCT), and by FCT/MCTES through the projects LA/P/0008/2020 (Doi:10.54499/LA/P/0008/2020), UIDP/50006/2020 (Doi: 10.54499/UIDP/50006/2020), and UIDB/50006/2020 (Doi:10.54499/UIDB/50006/2020), through national funds.
dc.identifier.citationPathogens. 2025 Jan 20;14(1):99. doi: 10.3390/pathogens14010099
dc.identifier.doi10.3390/pathogens14010099
dc.identifier.eissn2076-0817
dc.identifier.pmid39861060
dc.identifier.urihttp://hdl.handle.net/10400.18/10815
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationVeterinary and Animal Science Research Centre
dc.relationCentre for the Research and Technology of Agro-Environmental and Biological Sciences
dc.relationAssociate Laboratory for Animal and Veterinary Sciences
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationUIDB/50006/2020
dc.relation.hasversionhttps://www.mdpi.com/2076-0817/14/1/99
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectWildlife
dc.subjectKlebsiella spp.
dc.subjectRaoultella spp.
dc.subjectEnvironment
dc.subjectResistência aos Antimicrobianos
dc.subjectPortugal
dc.titleAssessment of Antibiotic Resistance Among Isolates of Klebsiella spp. and Raoultella spp. in Wildlife and Their Environment from Portugal: A Positive Epidemiologic Outcomeeng
dc.typejournal article
dcterms.referenceshttps://www.mdpi.com/article/10.3390/pathogens14010099/s1
dspace.entity.typePublication
oaire.awardTitleVeterinary and Animal Science Research Centre
oaire.awardTitleCentre for the Research and Technology of Agro-Environmental and Biological Sciences
oaire.awardTitleAssociate Laboratory for Animal and Veterinary Sciences
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00772%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04033%2F2020/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0008%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.citation.issue1
oaire.citation.startPage99
oaire.citation.titlePathogens
oaire.citation.volume14
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
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