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Next-generation sequencing and culture-based techniques offer complementary insights into fungi and prokaryotes in beach sands

2017-06-15Journal article Restricted access
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dc.contributor.authorRomão, Daniela
dc.contributor.authorStaley, Christopher
dc.contributor.authorFerreira, Filipa
dc.contributor.authorRodrigues, Raquel
dc.contributor.authorSabino, Raquel
dc.contributor.authorVeríssimo, Cristina
dc.contributor.authorWang, Ping
dc.contributor.authorSadowsky, Michael
dc.contributor.authorBrandão, João
dc.date.accessioned2018-02-27T17:33:55Z
dc.date.available2019-07-01T00:30:14Z
dc.date.issued2017-06-15
dc.description.abstractA next-generation sequencing (NGS) approach, in conjunction with culture-based methods, was used to examine fungal and prokaryotic communities for the presence of potential pathogens in beach sands throughout Portugal. Culture-based fungal enumeration revealed low and variable concentrations of the species targeted (yeasts and dermatophytes), which were underrepresented in the community characterized by NGS targeting the ITS1 region. Conversely, NGS indicated that the potentially pathogenic species Purpureocillium liliacinum comprised nearly the entire fungal community. Culturable fecal indicator bacterial concentrations were low throughout the study and unrelated to communities characterized by NGS. Notably, the prokaryotic communities characterized revealed a considerable abundance of archaea. Results highlight differences in communities between methods in beach sand monitoring but indicate the techniques offer complementary insights. Thus, there is a need to leverage culture-based methods with NGS methods, using a toolbox approach, to determine appropriate targets and metrics for beach sand monitoring to adequately protect public health.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMar Pollut Bull. 2017 Jun 15;119(1):351-358. doi: 10.1016/j.marpolbul.2017.04.036. Epub 2017 Apr 23.pt_PT
dc.identifier.doi10.1016/j.marpolbul.2017.04.036pt_PT
dc.identifier.issn0025-326X
dc.identifier.urihttp://hdl.handle.net/10400.18/5097
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://doi.org/10.1016/j.marpolbul.2017.04.036pt_PT
dc.subjectBacteriapt_PT
dc.subjectEnvironmental Monitoringpt_PT
dc.subjectFungipt_PT
dc.subjectPortugalpt_PT
dc.subjectBathing Beachespt_PT
dc.subjectHigh-Throughput Nucleotide Sequencingpt_PT
dc.subjectWater Microbiologypt_PT
dc.subjectÁgua e Solopt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleNext-generation sequencing and culture-based techniques offer complementary insights into fungi and prokaryotes in beach sandspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage358pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage351pt_PT
oaire.citation.titleMarine Pollution Bulletinpt_PT
oaire.citation.volume119pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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