Publication
Genetic Diversity of Salmonella enterica subsp. enterica Serovar Enteritidis from Human and Non-Human Sources in Portugal
| dc.contributor.author | Leão, Célia | |
| dc.contributor.author | Silveira, Leonor | |
| dc.contributor.author | Usié, Ana | |
| dc.contributor.author | Gião, Joana | |
| dc.contributor.author | Clemente, Lurdes | |
| dc.contributor.author | Themudo, Patricia | |
| dc.contributor.author | Amaro, Ana | |
| dc.contributor.author | Pista, Angela | |
| dc.date.accessioned | 2025-01-29T16:12:12Z | |
| dc.date.available | 2025-01-29T16:12:12Z | |
| dc.date.issued | 2024-01-26 | |
| dc.description | (This article belongs to the Special Issue Novel Insights into Pathogenesis and Antimicrobial Resistance of Salmonellae 2nd Edition) | |
| dc.description.abstract | Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) is one of the leading causes of foodborne infections associated with broilers and laying hens. Portugal has had the lowest notification rates of salmonellosis in recent years, due to the vaccinations of layer and breeder flocks and strict compliance with biosecurity measures. However, data about the genetic diversity of S. Enteritidis in Portugal are scarce. In this study, 102 S. Enteritidis isolates selected from human (n = 63) and non-human sources (n = 39) were characterized by serotyping, antimicrobial susceptibility, and whole genome sequencing. The S. Enteritidis population was mainly resistant to fluoroquinolones, and a sole isolate showed resistance to extended-spectrum cephalosporins. ST11 was the most frequent sequence type, and three novel STs from human isolates (ST9236, ST4457, and ST9995) were assigned. Several Salmonella pathogenic islands (SPI) and Putative SPI were present in the genomes, namely SPI-1, 2, 3, 4, 5, 9, 10, 12, 13, and 14, C63PI, CS54_island, and 170 virulence genes were identified. The phylogenetic analysis revealed that strains from Portugal are genetically heterogeneous regarding sample type, collection date, and genetic content. This study increases the available data, essential to a better characterization of strains in a global context. | eng |
| dc.description.sponsorship | This research study was carried out with the support of the ADONIS (Assessing Determinants of the Non-Decreasing Incidence of Salmonella), DISCOVER (Discovering the sources of Salmonella, Campylobacter, VTEC, and antimicrobial resistance), and OH-HARMONY CAP (One Health Harmonization of Protocols for the Detection of Foodborne Pathogens and AMR Determinants) projects, under the scope of the One Health consortium, grant agreement No 773830; and PTDC/CVTCVT/28469/2017 “CIAinVET—Food-producing animals as reservoirs of resistance to Critically Important Antibiotics” financed by the “Fundação para a Ciência e Tecnologia” (FCT). FCT for the Contrato–Programa was to A. Usié (https://doi.org/10.54499/CEECINST/00100/2021/CP2774/CT0001), and the financial support was to Research Units UIDB/05183/2020 (MED-Mediterranean Institute for Agriculture, Environment and Development) and CHANGE (LA/P/0121/2020). | |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Pathogens . 2024 Jan 26;13(2):112. doi: 10.3390/pathogens13020112. | |
| dc.identifier.doi | 10.3390/pathogens13020112 | pt_PT |
| dc.identifier.eissn | 2076-0817 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10319 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI | |
| dc.relation | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| dc.relation | Food-producing animals as reservoirs of resistance to Critically Important Antibiotics | |
| dc.relation.hasversion | https://www.mdpi.com/2076-0817/13/2/112 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Gastrointestinal Infections | |
| dc.subject | Infectious Diseases | |
| dc.subject | WGS | |
| dc.subject | Foodborne | |
| dc.subject | One Health | |
| dc.subject | Pathogenicity | |
| dc.subject | Salmonellosis | |
| dc.subject | Infecções Gastrointestinais | |
| dc.subject | Portugal | |
| dc.title | Genetic Diversity of Salmonella enterica subsp. enterica Serovar Enteritidis from Human and Non-Human Sources in Portugal | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| oaire.awardTitle | Food-producing animals as reservoirs of resistance to Critically Important Antibiotics | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/773830/EU | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-CVT%2F28469%2F2017/PT | |
| oaire.citation.issue | 2 | pt_PT |
| oaire.citation.startPage | 112 | pt_PT |
| oaire.citation.title | Pathogens | pt_PT |
| oaire.citation.volume | 13 | pt_PT |
| oaire.fundingStream | H2020 | |
| oaire.fundingStream | 3599-PPCDT | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | European Commission | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | 94d118fb-33ce-49fa-b1ed-d5bddf63581d | |
| relation.isProjectOfPublication | 274ed6bf-8e05-48f4-b02a-1aa7078cea43 | |
| relation.isProjectOfPublication.latestForDiscovery | 94d118fb-33ce-49fa-b1ed-d5bddf63581d |