Publication
Development of an orange juice in-house reference material and its application to guarantee the quality of vitamin C determination in fruits, juices and fruit pulps
| dc.contributor.author | Valente, A. | |
| dc.contributor.author | Sanches-Silva, A. | |
| dc.contributor.author | Albuquerque, T.G. | |
| dc.contributor.author | Costa, H.S. | |
| dc.date.accessioned | 2015-01-30T15:18:42Z | |
| dc.date.available | 2015-01-30T15:18:42Z | |
| dc.date.issued | 2014-07-01 | |
| dc.description.abstract | Reference materials are useful for the quality control of analytical procedures and to evaluate the performance of laboratories. There are few and expensive certified reference materials commercially available for vitamin C or ascorbic acid analysis in food matrices. In this study, the preparation and the suitability assessment of an orange juice in-house reference material (RM) for vitamin C analysis in fruits, juices and in fruit pulps is described. This RM was used for the development and full validation of an HPLC method. The results showed excellent linearity (r2 = 0.9995), good accuracy (96.6–97.3%) and precision, as relative standard deviation, ranged from 0.70% to 3.67%. The in-house RM was homogenous and stable at storage conditions (−80 °C) during 12 months. According to our results, this in-house RM is an excellent tool to use in quality control and method verification purposes for vitamin C analysis of fruits, juices and fruit pulps matrices. Furthermore, a stabilization solution with perchloric and metaphosphoric acids was developed which prevents degradation of ascorbic acid for a period of 12 months at −80 °C. | por |
| dc.description.sponsorship | O presente trabalho insere-se no projeto “Compostos bioativos e os seus benefícios para a saúde” (2012DAN730), financiado pelo INSA, I.P. Tânia Gonçalves Albuquerque agradece a Bolsa de Investigação Científica Ricardo Jorge (BRJ/DAN-2012) financiada pelo INSA, I.P. | por |
| dc.identifier.citation | Food Chem. 2014 Jul 1;154:71-7. doi: 10.1016/j.foodchem.2013.12.053 | por |
| dc.identifier.doi | 10.1016/j.foodchem.2013.12.053 | |
| dc.identifier.issn | 0308-8146 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/2728 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.relation | Compostos bioativos e os seus benefícios para a saúde (2012DAN730) | por |
| dc.relation.publisherversion | hhttp://www.sciencedirect.com/science/article/pii/S0308814613019195 | por |
| dc.subject | Composição dos Alimentos | por |
| dc.subject | Nutrição Aplicada | por |
| dc.subject | Ascorbic Acid | por |
| dc.subject | Vitamin C | por |
| dc.subject | Reference Material | por |
| dc.subject | Quality Control | por |
| dc.subject | Validation | por |
| dc.title | Development of an orange juice in-house reference material and its application to guarantee the quality of vitamin C determination in fruits, juices and fruit pulps | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 77 | por |
| oaire.citation.startPage | 71 | por |
| oaire.citation.title | Food Chemistry | por |
| oaire.citation.volume | 154 | por |
| rcaap.rights | embargoedAccess | por |
| rcaap.type | article | por |