Evaluation of antioxidant activity of seed, pulp and peel from Annona Cherimola Mill. (Annonaceae)

Santos, F.; Albuquerque, T.G.; Oliveira, M.B.; Sanches-Silva, A.; Costa, H.S.

http://hdl.handle.net/10400.18/1921

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Background and Objectives: The antioxidant compounds of fruits are very important when we want to prevent several diseases, such as cardiovascular diseases or even cancer. In this study, the antioxidant capacity of pulp, seeds and peel of ripe fruits of Annona cherimola Mill. was determined with two different methods, DPPH and Folin-Ciocalteu. Methods: Annona cherimola Mill. fruits were acquired in local supermarkets. Each part of the fruits (peel, seeds and pulp) was homogenised. For DPPH assay, three extracts were prepared: ethanolic, methanolic and aqueous. The ethanolic extract was also applied to ascorbic acid, BHT and gallic acid, which were employed as controls. The total phenolic concentration was measured using the Folin-Ciocalteu method and gallic acid as standard. Results: For DPPH assay, from the three solvents tested, aqueous extracts showed the lowest antioxidant activity for pulp and peel. In the case of peel, the EC50 is about half with methanol than with ethanol while the opposite was observed in the case of seeds. EC50 was similar for both organic solvents in the case of pulp. The synthetic antioxidants used as reference were more effective DPPH radical scavengers than all of the studied extracts. Conclusions: The two different methods showed that pulp has a lower antioxidant activity, when compared with peel and seeds. None of the extracts were as effective DPPH radical scavengers as the standard controls, vitamin C, gallic acid and BHT. These results will contribute to preserve biodiversity and promote several cultivars of traditional fruits from Madeira’s island.