Logo do repositório
 
Publicação

Micronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smoke

2025-12-05Artigo científico Acesso aberto
Ver registo simples
dc.contributor.authorEsteves, Filipa
dc.contributor.authorMadureira, Joana
dc.contributor.authorBarros, Bela
dc.contributor.authorAlves, Sara
dc.contributor.authorVaz, Josiana
dc.contributor.authorOliveira, Marta
dc.contributor.authorSlezakova, Klara
dc.contributor.authorFernandes, Adília
dc.contributor.authordo Carmo Pereira, Maria
dc.contributor.authorMorais, Simone
dc.contributor.authorBonassi, Stefano
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorCosta, Solange
dc.date.accessioned2026-02-23T10:20:03Z
dc.date.available2026-02-23T10:20:03Z
dc.date.issued2025-12-05
dc.description.abstractOccupational exposure as a firefighter is classified as carcinogenic to humans. However, information on the biological effects of wildland firefighting remains limited. This study aimed to assess genotoxicity in a group of wildland firefighters and evaluate the contribution of total concentration of urinary hydroxylated polycyclic aromatic hydrocarbons (ΣOHPAHs) to selected endpoints. A group of 59 northern Portuguese wildland firefighters (mean age: 35.5 ± 9.0 years) was evaluated before and during the wildfire season. Sociodemographic, lifestyle, occupational-related information was collected via questionnaire. The micronucleus (MN) assay in buccal and urothelial cells was applied to paired samples to assess the association with exposure, as indicated by urinary ΣOHPAHs levels. The risk of MN formation in urothelial cells was over twofold higher during the wildfire season [Frequency Ratio (FR): 2.13, 95 % CI: 1.99-2.27, p = 0.01]. A 35 % increase in MN frequency in buccal cells was observed during the wildfire season (FR: 1.35, 95 % CI: 0.76-2.40, p > 0.05). Urinary ΣOHPAHs exhibited a significant positive association with MN‰ in urothelial cells (FR:1.04, 95 % CI: 1.01-1.08, p < 0.05). This study provides the first assessment of MN frequency in urothelial cells of wildland firefighters, offering novel evidence of genotoxic risks and potential long-term health impacts associated with wildland firefighting.por
dc.description.abstractHighlights: - Micronucleus (MN) frequency is considered a potential early cancer risk biomarker. - MN assay in buccal and urothelial cells is minimally invasive. - MN‰ in firefighters’ urothelial cells were over 2x higher during wildfire season. - Urinary total OHPAHs significantly increased MN‰ in firefighters’ urothelial cells. - MN assay in urothelial cells may serve as a biomonitoring tool for firefighters.eng
dc.description.sponsorshipThis work received financial support from the project PCIF/SSO/0017/2018 (https://doi.org/10.54499/PCIF/SSO/0017/2018) by the Fundação para a Ciência e a Tecnologia (FCT), Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), through national funds. This work is financed by national funds through the FCT within the scope of projects UIDB/04750/2020 (https://doi.org/10.54499/UIDB/04750/2020) and LA/P/0064/2020 (https://doi.org/10.54499/LA/P/0064/2020). Filipa Esteves was supported by National Funds through FCT, under the Ph.D. fellowship UI/BD/150783/2020. The work of Stefano Bonassi was supported by the Italian Ministry of Health (Ricerca Corrente), Rome, Italy, and by the Competitive Funding for University Research Projects [Finanziamento competitivo di progetti di ricerca di ateneo (FIN/RIC)] San Raffaele University, Rome, Italy.
dc.identifier.citationJ Hazard Mater. 2025 Dec 5:500:140427. doi: 10.1016/j.jhazmat.2025.140427. Epub 2025 Nov 8
dc.identifier.doi10.1016/j.jhazmat.2025.140427
dc.identifier.issn0304-3894
dc.identifier.pmid41223470
dc.identifier.urihttp://hdl.handle.net/10400.18/10979
dc.language.isoen
dc.peerreviewedyes
dc.relationA panel of (bio)markers for the surveillance of firefighter's health and safety.
dc.relationEpidemiology Research Unit - Institute of Public Health, University of Porto
dc.relationLaboratory for Integrative and Translational Research in Population Health
dc.relationUI/BD/150783/2020
dc.relation.hasversionhttps://www.sciencedirect.com/science/article/pii/S0304389425033473?via%3Dihub
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectChromosomal Damage
dc.subjectDNA Damage
dc.subjectMicronucleus Assay
dc.subjectWildfire Smoke
dc.subjectWildland Firefighters
dc.subjectGenotoxicidade Ambiental
dc.subjectAr e Saúde Ocupacional
dc.titleMicronucleus assay in buccal and urothelial epithelial cells of wildland firefighters exposed to wildfire smokeeng
dc.typejournal article
dcterms.referenceshttps://ars.els-cdn.com/content/image/1-s2.0-S0304389425033473-mmc1.docx
dspace.entity.typePublication
oaire.awardNumberPCIF/SSO/0017/2018
oaire.awardNumberUIDB/04750/2020
oaire.awardNumberLA/P/0064/2020
oaire.awardNumberUI/BD/150783/2020
oaire.awardTitleA panel of (bio)markers for the surveillance of firefighter's health and safety.
oaire.awardTitleEpidemiology Research Unit - Institute of Public Health, University of Porto
oaire.awardTitleLaboratory for Integrative and Translational Research in Population Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de Projetos de Investigação Científica e Desenvolvimento Tecnológico no Âmbito da Prevenção e Combate a Incêndios Florestais - 2018/PCIF%2FSSO%2F0017%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04750%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0064%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/UI%2FBD%2F150783%2F2020/PT
oaire.citation.startPage140427
oaire.citation.volume500
oaire.fundingStreamConcurso de Projetos de Investigação Científica e Desenvolvimento Tecnológico no Âmbito da Prevenção e Combate a Incêndios Florestais - 2018
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_NORTE
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublicatione03b6804-4e26-4c3d-9cc7-8d614b5d4333
relation.isProjectOfPublication05f4f337-eeba-4c07-82ed-4202b21618a2
relation.isProjectOfPublication18ca3cb0-ef66-4dd8-b45e-4cb0654cd7e8
relation.isProjectOfPublicationc67e4748-32c2-442b-8b64-0748a80fefe8
relation.isProjectOfPublication.latestForDiscoverye03b6804-4e26-4c3d-9cc7-8d614b5d4333

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
1-s2.0-S0304389425033473-main.pdf
Tamanho:
1.25 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
4.03 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

DSA - Artigos em revistas internacionais
INSA - Artigos em revistas internacionais