Repository logo
 
Publication

Molecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samples

2020-01-10Journal article Open access
Simple item page
dc.contributor.authorSabino, Raquel
dc.contributor.authorSimões, Helena
dc.contributor.authorVeríssimo, Cristina
dc.date.accessioned2021-03-09T15:42:04Z
dc.date.available2021-03-09T15:42:04Z
dc.date.issued2020-01-10
dc.descriptionBrief Reportpt_PT
dc.description.abstractDiagnosis of invasive fungal infections is complex, and the lack of standardization of molecular methods is still a challenge. Several methods are available for the diagnosis of invasive aspergillosis, but their effectiveness will depend on the studied population, the patients' comorbidities, and the use of mold active prophylaxis, among others. The ability to determine the identity of the infecting Aspergillus species, and to detect mutations conferring specific resistance patterns directly from DNA extracted from the biological product, is an advantage of nucleic acid testing compared with antigen-based assays. In this study, we to present laboratory cases where the diagnosis of aspergillosis was performed using a real-time multiplex PCR for the detection of Aspergillus DNA in tissue samples, showing its usefulness as one more tool in the diagnosis of aspergillosis in tissue samples. Aspergillus real-time multiplex PCR was also used to detect azole-resistance in some cases. In the majority of the PCR positive cases, cultures remained negative after 60 days. The PCR assay directed to Aspergillus gave positive signals for Aspergillus fumigatus sensu stricto. Results were confirmed by panfungal PCR, followed by sequencing, revealing 100% homology with Aspergillus fumigatus sensu stricto. Mutations conferring azole resistance were not detected.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Fungi (Basel). 2020 Jan 10;6(1):11. doi: 10.3390/jof6010011.pt_PT
dc.identifier.doi10.3390/jof6010011pt_PT
dc.identifier.issn2309-608X
dc.identifier.urihttp://hdl.handle.net/10400.18/7372
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2309-608X/6/1/11pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAspergilluspt_PT
dc.subjectInvasive Fungal Infectionspt_PT
dc.subjectReal-time PCRpt_PT
dc.subjectMolecular Diagnosispt_PT
dc.subjectAzole-resistancept_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleMolecular Detection of Aspergillus: Application of a Real-Time PCR Multiplex Assay in Tissue Samplespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.conferencePlaceBasel, Switzerlandpt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage11pt_PT
oaire.citation.titleJournal of Fungipt_PT
oaire.citation.volume6pt_PT
rcaap.embargofctAcesso de acordo com página web do editor da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Molecular Detection of Aspergillus - Artigo publicado.pdf
Size:
240.58 KB
Format:
Adobe Portable Document Format
Download

Collections

DDI - Artigos em revistas internacionais