Logo do repositório
 
Publicação

Characterization of an Internal Ribosome Entry Site (IRES) in p53 mRNA

2017-05-30Documento de conferência Acesso restrito
Ver registo simples
dc.contributor.authorNeves, Ana Rita
dc.contributor.authorLacerda, Rafaela
dc.contributor.authorMarques-Ramos, Ana
dc.contributor.authorRomão, Luísa
dc.contributor.authorMatsuda, M.
dc.contributor.authorCandeias, Marco
dc.date.accessioned2018-03-05T12:31:30Z
dc.date.embargo2025-12-31
dc.date.issued2017-05-30
dc.description.abstractThe tumour suppressor p53 gene is one of the most studied cancer-related genes. So far, many p53 isoforms have been identified either resulting from alternative splicing or from non-canonical translation mechanisms. It is known that cap-dependent translation is repressed under stress conditions to preserve energy. Therefore, other translational mechanisms are required to keep the synthesis of vital proteins. Internal Ribosome Entry Sites (IRESes) were first discovered in viruses, and then observed in eukaryotes, as secondary structures present in RNA that were capable of recruiting ribosomes to the vicinity of an initiation codon inserted in an optimal environment allowing cap-independent translation of mRNAs. Translation of Δ40p53, a p53 isoform, is one example of this non-canonical mechanism due to the presence of an IRES near an alternative initiation codon (AUG40). Here, we will present and characterize a new IRES in p53 mRNA. We present details on the localization, structure, function and regulation of this IRES under normal and stress conditions. Importantly, our data reveals that the function of this IRES is required for cell survival and proliferation under certain cell conditions. This finding can have grave implications for understanding p53 function dynamics and cancer progression in specific environments.pt_PT
dc.description.sponsorshipFCT/PTDC/BIM-ONC/4890/2014pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/5161
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectCancerpt_PT
dc.subjectInternal Ribosome Entry Site (IRES)pt_PT
dc.subjectmRNA Translationpt_PT
dc.subjectGenómica Funcional e Estruturalpt_PT
dc.subjectExpressão Génicapt_PT
dc.titleCharacterization of an Internal Ribosome Entry Site (IRES) in p53 mRNApt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePrague, Czech Republicpt_PT
oaire.citation.title22nd Annual Meeting of the RNA Society, 30 May - 3 June 2017pt_PT
rcaap.embargofctOs resultados ainda não foram publicadospt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typeconferenceObjectpt_PT

Ficheiros

Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

DGH - Posters/abstracts em congressos internacionais