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Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)

2020-02-27Journal article Open access
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dc.contributor.authorBrandão, Fátima
dc.contributor.authorFernández-Bertólez, Natalia
dc.contributor.authorRosário, Fernanda
dc.contributor.authorBessa, Maria João
dc.contributor.authorFraga, Sónia
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorLaffon, Blanca
dc.contributor.authorValdiglesias, Vanessa
dc.contributor.authorCosta, Carla
dc.date.accessioned2021-03-04T10:57:53Z
dc.date.available2021-03-04T10:57:53Z
dc.date.issued2020-02-27
dc.descriptionThis article belongs to the Special Issue Toxicity and Ecotoxicity Assessment of Nanomaterials by In Vitro Modelspt_PT
dc.description.abstractTitanium dioxide nanoparticles (TiO2 NPs) have a wide variety of applications in many consumer products, including as food additives, increasing the concern about the possible hazards that TiO2 NPs may pose to human health. Although most previous studies have focused on the respiratory system, ingestion must also be considered as an important exposure route. Furthermore, after inhalation or ingestion, TiO2 NPs can reach several organs, such as the liver, brain or lungs. Taking this into consideration, the present study focuses on the uptake and potential genotoxicity (micronuclei induction) of TiO2 NPs on four human cell lines of diverse origin: lung cells (A549), liver cells (HepG2), glial cells (A172) and neurons (SH-SY5Y), using flow cytometry methods. Results showed a concentration-, time- and cell-type- dependent increase in TiO2 NPs uptake but no significant induction of micronuclei in any of the tested conditions. Data obtained reinforce the importance of cell model and testing protocols choice for toxicity assessment. However, some questions remain to be answered, namely on the role of cell culture media components on the agglomeration state and mitigation of TiO2 NPs toxic effects.pt_PT
dc.description.sponsorshipThe present work was supported by Xunta de Galicia (reference ED431B 2019/02), NanoBioBarriers (PTDC/MED-TOX/31162/2017) funded by Operational Program for Competitiveness and Internationalisation through European Regional Development Funds (FEDER/FNR) and through national funds by the Portuguese Foundation for Science and Technology (FCT) and NanoLegaTox (PTDC/SAU-PUB/29651/2017) project co-financed by COMPETE 2020, Portugal 2020 and European Union, through FEDER. F.B. are M.J.B. recipients of FCT fellowships (SFRH/BD/101060/2014 and SFRH/BD/12046/2016); V.V. is supported by a Beatriz Galindo Research Fellowship (reference BEAGAL18/00142); and NF-B by a Deputación Provincial da Coruña fellowship (2019000011550).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanomaterials (Basel). 2020 Feb 27;10(3):412. doi: 10.3390/nano10030412pt_PT
dc.identifier.doi10.3390/nano10030412pt_PT
dc.identifier.issn2079-4991
dc.identifier.urihttp://hdl.handle.net/10400.18/7302
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationSFRH/BD/12046/2016pt_PT
dc.relationEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
dc.relation.publisherversionhttps://www.mdpi.com/2079-4991/10/3/412pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectA172pt_PT
dc.subjectA549pt_PT
dc.subjectHepG2pt_PT
dc.subjectSH-SY5Ypt_PT
dc.subjectMicronucleipt_PT
dc.subjectTitanium Dioxide Nanoparticlespt_PT
dc.subjectTiO2 NPspt_PT
dc.subjectUptakept_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleGenotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-TOX%2F31162%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FSAU-PUB%2F29651%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F101060%2F2014/PT
oaire.citation.issue3pt_PT
oaire.citation.startPage412pt_PT
oaire.citation.titleNanomaterialspt_PT
oaire.citation.volume10pt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1370de00-d228-47a7-8aed-320831dc4dcd
relation.isProjectOfPublication9f23e866-c585-4ebc-af90-ee03cf2d2655
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relation.isProjectOfPublication.latestForDiscovery8bcb6d0f-fda0-470a-906d-c67c257beedb

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