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A comparative test of ixodid tick identification by a network of European researchers

2017-06Journal article Restricted access
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dc.contributor.authorEstrada-Peña, A.
dc.contributor.authorD'Amico, G.
dc.contributor.authorPalomar, A.M.
dc.contributor.authorDupraz, M.
dc.contributor.authorFonville, M.
dc.contributor.authorHeylen, D.
dc.contributor.authorHabela, M.A.
dc.contributor.authorHornok, S.
dc.contributor.authorLempereur, L.
dc.contributor.authorMadder, M.
dc.contributor.authorNúncio, M.S.
dc.contributor.authorOtranto, D.
dc.contributor.authorPfaffle, M.
dc.contributor.authorPlantard, O.
dc.contributor.authorSantos-Silva, M.M.
dc.contributor.authorSprong, H.
dc.contributor.authorVatansever, Z.
dc.contributor.authorVial, L.
dc.contributor.authorMihalca, A.D.
dc.date.accessioned2018-03-07T13:30:19Z
dc.date.available2018-03-07T13:30:19Z
dc.date.issued2017-06
dc.description.abstractThis study reports the results of a comparative test of identification of ticks occurring in Western Europe and Northern Africa. A total of 14 laboratories were voluntarily enrolled in the test. Each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, Hyalomma lusitanicum, Hy. marginatum, Ixodes ricinus, I. hexagonus, Rhipicephalus annulatus, R. bursa, R. rossicus, and/or R. sanguineus s.l. Ticks were morphologically identified by three of the co-authors and the identification confirmed by a fourth co-author who used molecular methods based on several genes. Then ticks were randomly selected and blindly distributed among participants, together with a questionnaire. Only specimens collected while questing and, if possible, in the same survey, were circulated. Because of the random nature of the test, a participant could receive several specimens of the same species. Species in the different genera had variable misidentification rates (MR) of 7% (Dermacentor), 14% (Ixodes), 19% (Haemaphysalis), 36% (Hyalomma), and 54% (Rhipicephalus). Within genera, the MR was also variable ranging from 5.4% for I. ricinus or 7.4% for D. marginatus or D. reticulatus to 100% for R. rossicus. The test provided a total misidentification rate of 29.6% of the species of ticks. There are no significant differences in MR according to the sex of the tick. Participants were requested to perform a second round of identifications on the same set of ticks, using only purposely prepared keys (without illustrations), circulated to the enrolled participants, including 2 species of the genus Dermacentor, 8 of Haemaphysalis, 10 of Hyalomma, 23 of Ixodes, and 6 of Rhipicephalus. The average MR in the second round was 28%: 0% (Dermacentor), 33% (Haemaphysalis), 30% (Hyalomma) 18% (Ixodes), and 50% (Rhipicephalus). Species which are not reported in the countries of a participating laboratory had always highest MR, i.e. purely Mediterranean species had highest MR by laboratories in Central and Northern Europe. Participants expressed their concerns about a correct identification for almost 50% of the ticks of the genera Hyalomma and Rhipicephalus. The results revealed less than total confidence in identifying the most prominent species of ticks in the Western Palearctic, and underpin the need for reference libraries for specialists involved in this task. Results also showed that a combination of certain genes may adequately identify the target species of ticks.pt_PT
dc.description.sponsorshipThis study has been carried out under the umbrella of the European COST Action TD1303, “EurNegVec”.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTicks Tick Borne Dis. 2017 Jun;8(4):540-546. doi: 10.1016/j.ttbdis.2017.03.001. Epub 2017 Mar 8pt_PT
dc.identifier.doi10.1016/j.ttbdis.2017.03.001pt_PT
dc.identifier.issn1877-959X
dc.identifier.urihttp://hdl.handle.net/10400.18/5301
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://doi.org/10.1016/j.ttbdis.2017.03.001pt_PT
dc.subjectComparative Testpt_PT
dc.subjectIdentificationpt_PT
dc.subjectIxodid Tickspt_PT
dc.subjectMolecularpt_PT
dc.subjectMorphologypt_PT
dc.subjectWestern Palearcticpt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleA comparative test of ixodid tick identification by a network of European researcherspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage546pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage540pt_PT
oaire.citation.titleTicks and Tick-borne Diseasespt_PT
oaire.citation.volume8pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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