Publication
Application of Fourier transform infrared spectroscopy and chemometrics for differentiation of Salmonella enterica serovar Enteritidis phage types
| dc.contributor.author | Preisner, Ornella | |
| dc.contributor.author | Guiomar, Raquel | |
| dc.contributor.author | Machado, Jorge | |
| dc.contributor.author | Menezes, José Cardoso | |
| dc.contributor.author | Lopes, João Almeida | |
| dc.date.accessioned | 2012-02-27T11:49:56Z | |
| dc.date.available | 2012-02-27T11:49:56Z | |
| dc.date.issued | 2010-06 | |
| dc.description.abstract | Fourier transform infrared (FT-IR) spectroscopy and chemometric techniques were used to discriminate five closely related Salmonella enterica serotype Enteritidis phage types, phage type 1 (PT1), PT1b, PT4b, PT6, and PT6a. Intact cells and outer membrane protein (OMP) extracts from bacterial cell membranes were subjected to FT-IR analysis in transmittance mode. Spectra were collected over a wavenumber range from 4,000 to 600 cm 1. Partial least-squares discriminant analysis (PLS-DA) was used to develop calibration models based on preprocessed FT-IR spectra. The analysis based on OMP extracts provided greater separation between the Salmonella Enteritidis PT1-PT1b, PT4b, and PT6-PT6a groups than the intact cell analysis. When these three phage type groups were considered, the method based on OMP extract FT-IR spectra was 100% accurate. Moreover, complementary local models that considered only the PT1-PT1b and PT6-PT6a groups were developed, and the level of discrimination increased. PT1 and PT1b isolates were differentiated successfully with the local model using the entire OMP extract spectrum (98.3% correct predictions), whereas the accuracy of discrimination between PT6 and PT6a isolates was 86.0%. Isolates belonging to different phage types (PT19, PT20, and PT21) were used with the model to test its robustness. For the first time it was demonstrated that FT-IR analysis of OMP extracts can be used for construction of robust models that allow fast and accurate discrimination of different Salmonella Enteritidis phage types. | por |
| dc.identifier.citation | Appl Environ Microbiol. 2010 Jun;76(11):3538-44. Epub 2010 Apr 2 | por |
| dc.identifier.issn | 0099-2240 | |
| dc.identifier.other | doi: 10.1128/AEM.01589-09 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/644 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | American Society for Microbiology | por |
| dc.relation.publisherversion | http://aem.asm.org/content/76/11/3538.long | por |
| dc.subject | Salmonella | por |
| dc.subject | FT-IR | por |
| dc.subject | Spectroscopy | por |
| dc.subject | Chemometric | por |
| dc.subject | Salmonella Enteritidis | por |
| dc.subject | Phage type | por |
| dc.subject | Infecções Gastrointestinais | por |
| dc.title | Application of Fourier transform infrared spectroscopy and chemometrics for differentiation of Salmonella enterica serovar Enteritidis phage types | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 3544 | por |
| oaire.citation.startPage | 3538 | por |
| oaire.citation.title | Applied and Environmental Microbiology | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
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